Sequences of polymerase chain reaction (PCR) primers and probes used for real time detection PCR analysis
| cDNA | Primer |
|---|---|
| Mdr1a | |
| Sense | 5′-GCA GGT TGG CTG GAC AGA TT-3′ |
| Antisense | 5′-GGA GCG CAA TTC CAT GGA TA-3′ |
| Probe | 5′ FAM-CCG CCA GAG TTC CCA GCA GCA TG-TAMRA 3′ |
| Mdr1b | |
| Sense | 5′-AAA CAT GGC ACG TAA CCA AAG TT-3′ |
| Antisense | 5′-AAA ATG TGG CCC TGT TTA ATG ATT-3′ |
| Probe | 5′ FAM-CAC TGT TAA AGG TAA TTT CAT CAA GAC GAG AAG CCT TC-TAMRA 3′ |
| Mdr2 | |
| Sense | 5′-AGT TCA CGG GCG CAT CAA-3′ |
| Antisense | 5′-AAA AGA CAC TGG TGG CAC GTT-3′ |
| Probe | 5′ FAM-CAT CAA GTT CAT TGG TTT CCA CAT CCA GC-TAMRA 3′ |
| Bsep | |
| Sense | 5′-CCA AGC TGC CAA GGA TGC TA-3′ |
| Antisense | 5′-CCT TCT CCA ACA AGG GTG TCA-3′ |
| Probe | 5′ FAM-CAT TAT GGC CCT GCC GCA GCA-TAMRA 3′ |
| Mrp1 | |
| Sense | 5′-GCC ACT GCC TCA TGC CTA TT-3′ |
| Antisense | 5′-GCA AGA CCT GAA GGC AAG ATA CA-3′ |
| Probe | 5′ FAM-AGC CAC ATT TAT AGA GCC AAG CCA GAG CC-TAMRA 3′ |
| Mrp2 | |
| Sense | 5′-GAC GAC GAT GAT GGG CTG AT-3′ |
| Antisense | 5′-CTT CTC ATG GCC AAG GAA GCT-3′ |
| Probe | 5′ FAM-CCC ACC ATG GAG GAA ATC CCT GAG G-TAMRA 3′ |
| Mrp3 | |
| Sense | 5′-TCC CAC TTC TCG GAG ACA GTA ACT-3′ |
| Antisense | 5′-CTT AGC ATC ACT GAG GAC CTT GAA-3′ |
| Probe | 5′ FAM-CAG TGT CAT TCG GGC CTA CGG CC-TAMRA 3′ |
| Mrp4 | |
| Sense | 5′-TCA GTG TTG GAC AGA GAC AGT TAG TG-3′ |
| Antisense | 5′-CTT CTC CCG GAT TTT CTG TTG TAT-3′ |
| Probe | 5′ FAM-TCA GTT CTC GGA TCC ACA TTT GCA GTT G-TAMRA 3′ |
| Mrp6 | |
| Sense | 5′-CTC TCC CAT TGG CTT CTT TGA G-3′ |
| Antisense | 5′-GTC CAC ATC CAC TAT GTC CGT CT-3′ |
| Probe | 5′ FAM-TCG GGA ACC TGC TGA ACC GTT TTT C-TAMRA 3′ |
| Abca1 | |
| Sense | 5′-CCC AGA GCA AAA AGC GAC TC-3′ |
| Antisense | 5′-GGT CAT CAT CAC TTT GGT CCT TG -3′ |
| Probe | 5′ FAM-AGA CTA CTC TGT CTC TCA GAC AAC ACT TGA CCA AG-TAMRA 3′ |
| Bcrp | |
| Sense | 5′-CAG GTA GGC AAT TGT GAG GAA GA-3′ |
| Antisense | 5′-AAT CAG GGC ATC GAT CTG TCA-3′ |
| Probe | 5′ FAM-CAT GCA AGC CAG GGC CAC ATG A-TAMRA 3′ |
| Thy1 | |
| Sense | 5′-GCA GAT GTC CCG AGG ACA GA-3′ |
| Antisense | 5′-GGC AGT CCA GTC GAA GGT TCT-3′ |
| Probe | 5′ FAM-CAG CCT GAC AGC CTG CCT GGT GA-TAMRA 3′ |
| 18S | |
| Sense | 5′-CGG CTA CCA CAT CCA AGG A-3′ |
| Antisense | 5′-CCA ATT ACA GGG CCT CGA AA-3′ |
| Probe | 5′ FAM-CGC GCA AAT TAC CCA CTC CCG A-TAMRA 3′ |