Primer summary
| Primer | Sequence 5′ to 3′ | Source |
|---|---|---|
| GAPDH, glyceraldehyde-3-phosphate dehydrogenase; COX-2, cyclooxygenase 2; iNOS, inducible nitric oxide; ICAM-1, intercellular adhesion molecule 1; IL, interleukin; TNF-α, tumour necrosis factor α. | ||
| GAPDH | ATGGCACAGTCAAGGCTGAGA | NM_017008 |
| CGCTCCTGGAAGATGGTGAT | ||
| IL-6 | GCCCTTCAGGAACAGCTATGA | M26744 |
| TGTCAACAACATCAGTCCCAAGA | ||
| IL-1β | CACCTCTAAGCAGAGCACAG | Li and Wang (Brain Res Brain Res Protoc 2000;5:211–17). |
| GGGTTCCATGGTGAAGTCAAC | ||
| TNF-α | GGTGATCGGTCCCAACAAGGA | Fink, et al (Nat Med 1998;4:1329–33). |
| CACGCTGGCTCAGCCACTC | ||
| ICAM-1 | CGTGGCGTCCATTTACACCT | NM_012967 |
| TTAGGGCCTCCTCCTGAGC | ||
| iNOS | GGAGAGATTTTTCACGACACCC | NM_012611 |
| CCATGCATAATTTGGACTTGCA | ||
| COX-2 | CTCTGCGATGCTCTTCCGAG | AF233596 |
| AAGGATTTGCTGCATGGCTG | ||
| IL-10 | TGCAACAGCTCAGCGCA | Harness, et al (J Neurol Sci 2001;187:7–16). |
| GTCACAGCTTTCGAGAGACTGGAA | ||