Table 3

Nucleotide sequences for assay primers and probes for polymorphisms in the SERT gene

Single nucleotide polymorphisms
Polymorphism	Assay type	Forward primer sequence 5′–3′	Reverse primer sequence 5′–3′	VIC probe sequence 5′–3′	Fam probe sequence 5′–3′
5HTT-T623C	TaqMan	TGC CTC TTC CCC GGT AGT G	ATC GCC ACA AGC ACC TTG TC	GCC CCG CCT CCA ATT CAG GT	GCC CCG CCT CCA GTT CAG GT
5HTT-T3287C	TaqMan	CAC CCC GCC CCG TAG	GCG CTC CCG CTG GAT	CCC TCC CTG GCG AGC GC	CCC TCC CCG GCG AGC G
5HTT-G674A	TaqMan	GAA TTC AGG ACT GGA TGC CTT C	AGA GGG TAG AAA ATG TGT GCA GGT A	CCT GAG AAC ACA CGT TGT CCT CAA AAA T	TCC TGA GAA CAC ACA TTG TCC TCA AAA ATC
5HTT-C867T	TaqMan	CCT CTC CCC AAA GTA TTG CTT CT	GCG TTC CCT GGC CTC TCT	CAT TTC CCT TCC GTA GAC CCT CTG G	CGC ATT TCC CTT CTG TAG ACC CTC TG
5HTT-A2631C	TaqMan	GCA TCA GTA ACC TGC ACA CTC TTC	CCA GCA ACT CCT GTG GCT AAG	TTG CTT GCC CTC TAT TGC AGA ATA ACA AG	TGC TTG CCC TCT CTT GCA GAA TAA CA
5HTT-G160A	TaqMan	TGT GTC AAA ACC TGA TTG ACA CAT AA	GTC CTA CAA AGA TGT CAC AGA GGA AA	TGA TGA GAA TTG TAA CTG TTG TTG T	TGA TGA GAA TTA TAA CTG TTG TTG TG
5HTT-G769T	TaqMan	CGT AGG AGA GAA CAG GGA TGC TA	CAG CAG CAC ATG GAT TAG AAG GT	TGA GTA GCA TAT AGA ATT TTA TTG CTG	CTG AGT AGC ATA TAT AAT TTT ATT GCT G

Other polymorphisms
Polymorphism	Assay type	Forward primer	Reverse primer
5-HTT, 5-hydroxtryptamine transporter ( = SERT); PCR, polymerase chain reaction; VNTR, variable number of tandem repeats.
5HTT-VNTR	PCR	GTC AGT ATC ACA GGC TGC GAG	TGT TCC TAG TCT TAC GCC AGT G
SERT-P	PCR	GAG GGA CTG AGC TGG ACA ACC AC	GGC GTT GCC GCT CTG AAT GC