Immunostains used in this study
| Antibody | Clone | Manufacturer and type | Retrieval | ||
|---|---|---|---|---|---|
| *Five cases with absent α-actin staining were confirmed by use of a further commercially available antibody and method of antigen retrieval. | |||||
| Vimentin | (Vim 3B4) | Dako | Monoclonal mouse antihuman | None | |
| Desmin | (D33) | Dako | Monoclonal mouse antihuman | None | |
| Smooth muscle α-actin | (1A4) | Dako | Monoclonal mouse antihuman | None | |
| (specificity against α-isoactin only) | |||||
| Muscle actin | (HHF35) | Dako | Monoclonal mouse antihuman | None | |
| (specificity against α- and γ-isoactin)) | |||||
| Sarcomeric actin | (α-Sr-1) | Dako | Monoclonal mouse antirabbit | None | |
| (specificity against sarcomeric actin only) | |||||
| Smooth muscle α-actin* | (1A4) | Dako | Monoclonal mouse antihuman | Microwave | |
| Smooth muscle α-actin* | (αsm-1) | NC | Monoclonal mouse antihuman | None | |