cDNA array overexpression ratio and quantitative real time RT-PCR* overexpression fold in malignant gastrointestinal stromal tumours (GISTs) compared with benign GISTs
| Gene | VIL2 | COL8 | CCNB1 | HMG2 | TSG101 | CENP-F | FAK | DYRK2 |
|---|---|---|---|---|---|---|---|---|
| GeneBank accession No | X51521 | X57527 | M25753 | X62534 | X13293 | U19769 | L13616 | Y13493 |
| Chromosomal locus | 6q25.3 | 3q12−q13.1 | 5q12 | 4q31 | 11p15 | 1q32–q41 | 8q24−qter | 12q13.13 |
| Gene expression alterations in six malignant GISTs (GIST 4–9) compared with expression of three benign GISTs (GIST 1–3) using cDNA arrays and quantitative real time reverse transcription-polymerase chain reaction (RT-PCR). | ||||||||
| *Standard deviation for microarray overexpression fold is shown in parentheses and quantitative real time RT-PCR overexpression is shown in square parentheses. | ||||||||
| VIL2, villin 2; COL8A1, collagen 8 alpha 1 subunit; CCNB1, G2/mitotic specific cyclin B1; HMG2, high mobility group protein; TSG101, TSG101 tumour susceptibility protein; CENP-F, CENP-F kinetochore protein; FAK, protein tyrosine kinase 2; DYRK2, protein kinase DYRK2. | ||||||||
| Sample | ||||||||
| GIST4 | 9.1 (2.3)) [8] | 3.4 (0.4)) [1.2] | 3.1 (0.4) [1.9] | 3.9 (1.1) [13.8] | 3.6 (1.2) [1.1] | 5.1 (1.7) [5.5] | 5.8 (0.7) [2.9] | 5.7 (0.5) [1.9] |
| GIST5 | 8.6 (3.1) [4.6] | 9.7 (0.6) [11.4] | 2.7 (0.9) [1] | 2.9 (0.9) [18.3] | 4.1 (0.5) [2.4] | 1 (0.3) [1.4] | 2.7 (0.2) [1] | 2.7 (0.4) [1.2] |
| GIST6 | 6.8 (1.4) [3.7] | 4.7 (0.8) [16] | 6.2 (1.5) [24.2] | 4.3 (1.7) [18.6] | 3.2 (1.4) [2.4] | 8.7 (2.7) [1.4] | 4.9 (1.4) [2.6] | 4.7 (0.8) [6.3] |
| GIST7 | 3.2 (1.1) [13.7] | 5.4 (1.1) [59.6] | 3.1 (1.5) [8.6] | 3.2 (0.4) [7] | 2.8 (0.6) [2.6] | 2.5 (1.9) [8.2] | 2.5 (0.6) [5.3] | 2.5 (0.5) [5.7] |
| GIST8 | 9.8 (1.3) [13.7] | 4.3 (0.9) [9.2] | 6.1 (1.8) [12.1] | 3.1 (0.4) [1.9] | 2 (0.4) [1.5] | 3.8 (1.1) [11.1] | 5.2 (1.2) [1.9] | 3.6 (0.7) [1.2] |
| GIST9 | 2.6 (0.7) [1.5] | 0.8 (0.2) [0] | 3.1 (0.8) [13.0] | 4.7 (1.3) [6.4] | 3.8 (0.6) [1.8] | 4.8 (1.2) [10.9] | 3.5 (0.7) [2.3] | 1.3 (0.8) [1.7] |