List of primers for determination of the methylation status of methylated in tumours (MINTs) and genes by combined bisulphite restriction analysis and methylation specific polymerase chain reaction
| Gene/MINT | Forward primer/restriction enzyme | Reverse primer | Ref |
|---|---|---|---|
| p14ARF (unmethylated) | 5′TTTTTGGTGTTAAAGGGTGGTGTAGT | 5′CACAAAAACCCTCACTCACAACAA | 35 |
| p14ARF (methylated) | 5′GTGTTAAAGGGCGGCGTAGC | 5′AAAACCCTCACTCGCGACGA | |
| p16INK4A (unmethylated) | 5′TTATTAGAGGGTGGGGTGGATTGT | 5′CAACCCCAAACCACAACCATAA | 36 |
| p16INK4A (methylated) | 5′TTATTAGAGGGTGGGGCGGATGC | 5′GACCCCGAACCGCGACCGTAA | |
| MGMT (unmethylated) | 5′TTTGTGTTTTGATGTTTGTAGGTTTTTGT | 5′AACTCCACACTCTTCCAAAAACAAAACA | 37 |
| MGMT (methylated) | 5′TTTCGACGTTCGTAGGTTTTCGC | 5′GCACTCTTCCGAAAA CGAAACG | |
| HPP1 (1st set) | 5′GGCGTTTTGATTTTTTTTTTATTTTGTTTT | 5′AACGCGCTAAAATAAACTAATCTCTACTAA | 20 |
| HPP1 (nested) | 5′GTTTTTAGAGTTTTTTTTTTATGGTAGT RsaI at 37°C | 5′AACAATCACTTAAAAAAATAAAAAAACAA | |
| hMLH1 (1st set) | 5′TAGTAGTCGTTTTAGGAGGGA | 5′TCTAAATACTCAACGAAAATACCTT | 22 |
| hMLH1 (nested) | 5′GATTTAGTAATTTATAGAGT RsaI, 37°C | 5′AATACCTTCAACCAATCAC | 22 |
| MINT1 | 5′GGGTTGGAGAGTAGGGGAGTT TaqI, 65°C | 5′CGATCTAAAATTAGCTCGATAAGTTA | 15 |
| MINT2 (1st set) | 5′CGTTATGATTTTTTTGTTTAGTTAAT | 5′CAACACATTTTTCTAACACTTTC | 15 |
| MINT2 (nested) | 5′CGTTATGATTTTTTTGTTTAGTTAAT TaqI, 65°C | 5′TACAGCAACTAGCCAACTACCTC | |
| MINT12 (1st set) | 5′AGTTTTTAGTAAGGTTAGTGATTTAG | 5′ACCTAAATAAACTCCCAATTTA | 15 |
| MINT12 (nested) | 5′CGGGTTATGTTTTATTTTTTGTGTTT HpyCIV, 37°C | 5′CTCAAAAAAATCAAACAACCAACCAA | |
| MINT31 (1st set) | 5′TATATAATTTTGTGTATGGATT | 5′AACCTAATAAATCACTCAATTCC | 15 |
| MINT31 (nested) | 5′GACGGCGTAGTAGTTATTTGTT BstUI, 60°C | 5′CATCAGGAGGCCTCACTTTAC |