List of primers used for reverse transcription-polymerase chain reaction
| Gene name | Source | Forward primer (5′→3′) | Reverse primer (3′→5′) | Product size (bp) | Cycle No | |||
|---|---|---|---|---|---|---|---|---|
| *Rat EP1 primers were designed to generate no amplicons from either EP1 variant cDNA (unspliced EP1 mRNA, Genbank D88752) or genomic DNA.39 | ||||||||
| Mu, mouse; Hu, human. | ||||||||
| β-Actin | Mu | NM_007393 | AACACCCCAGCCATGTACG | (Exon 4) | CGCTCAGGAGGAGCAATGA | (Exon 6) | 623 | 22 |
| Rat | NM_031144 | AACACCCCAGCCATGTACG | (Exon 4) | CGCTCAGGAGGAGCAATGA | (Exon 6) | 623 | 18 | |
| Hu | NM_001101 | AACACCCCAGCCATGTACG | (Exon 4) | CGCTCAGGAGGAGCAATGA | (Exon 6) | 623 | 21 | |
| EP1 | Mu | NM_013641 | GACGATTCCGAAAGACCGCAG | (Exon 2) | CAACACCACCAACACCAGCAG | (Exon 2 to 3) | 242 | 32 |
| Rat* | D88751 | GAGAACGCAGGTCCCGATG | (Exon 1) | CCAACACCACCAATACCAGCAG | (Exon 1) | 232 | 35 | |
| Hu | NM_000955 | GGTATCATGGTGGTGTCGTG | (Exon 2) | GGCCTCTGGTTGTGCTTAGA | (Exon 3) | 317 | 40 | |
| EP2 | Mu | NM_008964 | GATGGCAGAGGAGACGGAC | (Exon 1) | ACTGGCACTGGACTGGGTAGA | (Exon 2) | 295 | 28 |
| Rat | NM_031088 | TGCTCATCGTGGCTGTGCTC | (Exon 1) | GCTCTCAGTGAAGTCCGACAAC | (Exon 2) | 394 | 35 | |
| Hu | NM_000956 | CCACCTCATTCTCCTGGCTA | (Exon 1) | CGACAACAGAGGACTGAACG | (Exon 2) | 216 | 34 | |
| EP3 | Mu | D10204 | TGCTGGCTCTGGTGGTGAC | (Exon 1) | ACTCCTTCTCCTTTCCCATCTGTG | (Exon 2) | 258 | 30 |
| Rat | D14869 | CCTTTGCCTCCGCCTTCG | (Exon 1) | CGAACGGCGATTAGGAAGG | (Exon 2) | 313 | 35 | |
| Hu | D38297 | CTTCGCATAACTGGGGCAAC | (Exon 1) | TCTCCGTGTGTGTCTTGCAG | (Exon 2) | 300 | 35 | |
| EP4 | Mu | BC011193 | CTGGTGGTGCTCATCTGCTC | (Exon 2) | AGGTGGTGTCTGCTTGGGTC | (Exon 3) | 445 | 30 |
| Rat | NM_032076 | GCCTCAGTGACTTTCGCCG | (Exon 1) | GCTGTGCTGAACCGTCTCTG | (Exon 2) | 336 | 35 | |
| Hu | NM_000958 | TGGTATGTGGGCTGGCTG | (Exon 2) | GAGGACGGTGGCGAGAAT | (Exon 3) | 329 | 35 | |