Table 2

List of primers used for real time reverse transcription-polymerase chain reaction

Gene name		Primer sequences (5′→3′)			Product size (bp)	Cycle condition
Mu, mouse; Hu, human.
β-Actin	Mu, Rat, Hu	Forward	CTACAATGAGCTGCGTGTG	(Exon 3)	122	95°C (20 s) → 60°C (20 s) → 72°C (10 s)
β-Actin	Mu, Rat, Hu	Reverse	TGGGGTGTTGAAGGTCTC	(Exon 4)	122	95°C (20 s) → 60°C (20 s) → 72°C (10 s)
EP₃	Mouse	Forward	GCTGTCCGTCTGTTGGTC	(Exon 1)	100	95°C (3 s) → 60°C (20 s)
EP₃	Mouse	Reverse	CCTTCTCCTTTCCCATCTG	(Exon 2)	100	95°C (3 s) → 60°C (20 s)
	Rat	Forward	ACTGTCCGTCTGCTGGTC	(Exon 1)	100	95°C (3 s) → 60°C (20 s)
	Rat	Reverse	CCTTCTCCTTTCCCATCTG	(Exon 2)	100	95°C (3 s) → 60°C (20 s)
	Human	Forward	GTGCTGTCGGTCTGCTG	(Exon 1)	102	95°C (3 s) → 66°C (20 s)
	Human	Reverse	CTTTCTGCTTCTCCGTGTG	(Exon 2)	102	95°C (3 s) → 66°C (20 s)