Primer sequences and restriction enzymes used for restriction fragment length polymorphism analysis of tumour necrosis factor A (TNF-A) and interleukin 10 (IL-10) polymorphisms. Typing of the remaining single nucleotide polymorphisms was performed by allelic discriminating TaqMan PCR, as described in materials and methods
| Target sequence | Sequences of primers and probes | Restriction enzyme | |
|---|---|---|---|
| FP, forward primer; RP, reverse primer. | |||
| TNF-A-307 | FP | 5′- GAG GCA ATA GGT TTT GAG GGC CAT-3′ | NcoI |
| RP | 5′- GGG ACA CAC AAG CAT CAA G-3′ | ||
| IL-10-592 | FP | 5′-ATC CAA GAC AAC ACT ACT AA-3′ | MaeIII |
| RP | 5′-TAA ATA TCC TCA AAG TTC C-3′ | ||
| IL-10-1082 | FP | 5′- TCG CTG CAA CCC AAC TGG C-3′ | MnlI |
| RP | 5′- GGT CCC TTA CTT TGC TCT TAC C-3′ | ||
| IL-10-819 | FP | 5′-GAC TCC AGC CAC AGA AGC TTA C-3′ | RsaI |
| RP | 5′-AGG TCT CTG GGC CTT AGT-3′ | ||