PGE2 released (pg/μg DNA) | p Value | ||
---|---|---|---|
−PAF | +PAF | ||
Stellate cells were isolated and cultured as described in the methods section. After overnight culture, cells were washed and placed in serum free Dulbecco’s modified Eagle’s medium containing 0.1% bovine serum albumin (BSA). Following a 15 minute equilibration period, cells were stimulated with vehicle (saline/0.25% BSA) or 100 nM PAF. After 15 minutes of incubation, the medium was aspirated and analysed for PGE2 content, as described in the methods sections. | |||
Results are means (SD) of triplicate determinations of an experiment repeated three times. | |||
Control | 0.18 (0.02) | 0.51 (0.11) | <0.01 |
Cirrhotic | 0.29 (0.07) | 1.27 (0.07) | <0.001 |
p Value | <0.05 | <0.001 |