Real time polymerase chain reaction results for DMT-1, IREG1, and HFE in total HH, treated HH, untreated HH, ID, and control groups
| Variable | Total HH group (n = 33) | HH untreated (n = 21) | HH treated (n = 12) | ID (n = 9) | Controls (n = 10) |
|---|---|---|---|---|---|
| Values are mean (SD). | |||||
| DMT1, divalent metal transporter 1; IREG1, iron regulated gene 1; HH, hereditary haemochromatosis; HH untreated, patients who had not yet undergone phlebotomy therapy; HH treated, patients who were iron depleted; ID, iron deficiency. | |||||
| *Results for DMT-1 and IREG1 were subsequently transformed logarithmically for statistical evaluation. | |||||
| Comparison of mean mRNA levels between cohorts revealed the following significant differences. For DMT1 mRNA: ID versus controls, p = 0.018; HH untreated versus HH treated, p = 0.002; HH treated versus controls, p = 0.006. For IREG1 mRNA: ID versus controls, p = 0.016. No significant differences in HFE mRNA levels were observed between any of the cohorts. | |||||
| DMT1* | 0.98 (1.7) | 0.44 (0.9) | 1.9 (2.3) | 4.0 (5.4) | 0.3 (0.3) |
| IREG1* | 0.96 (0.9) | 0.7 (0.5) | 1.4 (1.3) | 2.2 (1.7) | 0.79 (0.4) |
| HFE | 1.7 (1.1) n = 32 | 1.5 (1.0) n = 20 | 2.1 (1.3) | 3.4 (2.8) | 1.8 (1.1) |