Effect of leptin on cytokine production in RBhigh and RBlow cells
| IL-6 (pg/ml) | IL-10 (pg/ml) | |
|---|---|---|
| RBhigh and RBlow cells were prepared as described in materials and methods and stimulated overnight with anti-CD3 and anti-CD28, in the presence or absence of leptin. Cytokine levels (interleukin (IL)-6 and IL-10) were below detection limits in cells cultured in the absence of anti-CD3 and anti-CD28, with or without leptin. | ||
| Data are mean (SEM); n = 3. *p<0.05, **p<0.01 versus respective no leptin. | ||
| RBhigh–no leptin | 148.1 (22.7) | 249.5 (22.2) |
| RBhigh–with leptin | 78.5 (32.1)* | <10** |
| RBlow–no leptin | 42.4 (34.6) | 427.7 (15.7) |
| RBlow–with leptin | 29.9 (24.4) | 354.4 (52.2) |