Primer sequences and restriction enzymes used for SNP-genotyping
| SNP | Primers | Restriction enzyme | Length of restriction fragments |
|---|---|---|---|
| The underlined bases in the primers differ from the original sequences and served to introduce a restriction site or to disrupt a natural site within the primer sequence. | |||
| SLC22A4 1672C→T | F: CGTCATGGGTAGTCTGACTGTCCTGATTGGGATC | BamH I | allel C: 30 bp+88 bp |
| (rs1050152) | R: TCCTACTTACCATTTCACTTTCTGCATCTGCTCTAAGG | allel T: 118 bp | |
| SLC22A5 −207G→C | F: GCGCCGCTCTGCCTGCCAG | Msp I | allel G: 44 bp+83 bp |
| (rs2631367) | R: AGGGTAGGCTCGCGAGCTGACACC | allel C: 127 bp | |
| DLG5 113G→A | F: GGAAGGCGCAGTCCCCACCACCCCTCCTCAC | Msp I | allel G: 89 bp+35 bp |
| (rs1248696) | R: AAGGCCAGGCGCTTGCGGAGCTCGTTTCTCTCCTGG | allel A: 124 bp | |
| DLG5 4136 C→A | F: AGCTCACACCTGGACCCTGCCGGTAC | Xcm I | allel C: 127 bp+36 bp |
| (rs2289310) | R: TCACAGCAACGTCTGCTGACCTGGAGCTCCACTGC | allel A: 163 bp | |
| DLG5_e26 | F: CGACATCCTCTACGTGGATGACACCTTACC | Mwo I | insA: 156 bp+85 bp |
| R: AGATAAGAAGCAGAATCCCTCCTCCACCAGC | delA: 240 bp | ||
| IGR2078a_1 | F: TCACTGAGCACAGCTTCTACAGTGCCA | Hae III | allel A: 216 bp |
| R: GTTCCTAATCTGAACACAGAAGCCAGGGAG | allel G: 122 bp+94 bp | ||
| CARD15 2104C→T | F: TGGGGCCTGCTGGCTGAGTG | Msp I | allel C: 76 bp+45 bp |
| (rs2066844) | R: GTGCAGCTGGCGGGATGGAG | allel T: 121 bp | |
| CARD15 2722G→C | F: TCTGGCTGGGACTGCAGAGG | BstU I | allel G: 131 bp |
| (rs2066845) | R: CCCCTCGTCACCCACTCTGTCGC | allel C: 109 bp+22 bp | |
| CARD15 3020insC | F: GGCTAACTCCTGCAGTCTCTTTAACTGG | Mwo I | non-ins C: 168 bp |
| R: ACTTCCAGGATGGTGTCATTCCGCTCAAGG | insC: 143 bp+26 bp | ||