Table 1

Primers and conditions for polymerase chain reaction (PCR)

Transcript	Forward and reverse primers	Genbank accession No	No of cycles
All primer sequences are given as 5′-3′. Superscript numerals indicate the primer annealing positions with respect to the cDNA sequence deposited under the stated Genbank accession number.
Thermal cycling parameters for all PCR reactions were: 95°C five minutes followed by (94°C 30 seconds, 55°C 30 seconds, 72°C 90 seconds) for the stated number of cycles.
Symbols indicate where the cycle number was altered depending on the RNA source: *poly-A⁺ RNA from biopsies; †poly-A⁺ RNA from monocytes; ‡poly-A⁺ RNA from biopsies and monocytes; and §total RNA from Caco-2 cells.
SLC30A1	¹²⁵¹TCAGCCTGAATTTGCTAG¹²⁶⁸	NM_021194	30
	¹⁵¹⁴GATTCAGGTTGTTTGTTTGGC¹⁴⁹⁴
SLC30A4	⁷⁶³CTGACCTAAGCGCCATCATACTCACCC⁷⁸⁹	NM_013309	30
	¹¹⁴¹AATGCAGCTCTCACTGCC¹¹²⁴
SLC30A5	¹⁹⁴⁵GGTGGAGGCATGAATGCTA¹⁹⁶³	NM_022902	30
	²¹⁶⁶TTCTGGTGGCAATCTCAGG²¹⁴⁸
SLC39A1	⁴⁴⁷TGAGAGTCACTGGAGCTACCA⁴⁶⁷	NM_014437	30
	⁷³²AGGAGACAAGTGGCCAAAAA⁷¹³
SLC39A4	³²⁸GCCTGCGGGGTCAGGGCTGC³⁴⁷	NM_130849	30
	⁵¹⁸GGCCCGGGGTCAGGGCCTTG⁴⁹⁹
MT	⁷¹ATGGACCCCAACTGCTCC⁸⁸	NM_005946	*25, †30
	²⁵⁶TCAGGCACAGCAGCTGCAC²³⁸
β-actin	⁸⁹⁴TCCACGAAACTACCTTCAAC⁹¹³	NM_001101	‡25, §30
	¹⁴⁹⁸TTTAGGATGGCAAGGGAC¹⁴⁸¹