Proliferative responses of PBMC and LPL to PHA, OKT3, and β-galactosidase: [3H]-thymidine incorporation (cpm)
| PBMC | LPL | |||||
|---|---|---|---|---|---|---|
| HP+ve | HP−ve | p Value | HP+ve | HP−ve | p Value | |
| HP, Helicobacter pylori; PBMC, peripheral blood mononuclear cells; LPL, lamina propria lymphocytes; β-gal, β-galactosidase. | ||||||
| Results are expressed as [3H]-thymidine incorporation (cpm) into PBMC and LPL cultured for three days. LPL (4×105/ml) were cultured with autologous irradiated (2500 rads) PBMC (2×106/ml) in the presence of IL-2 (2 IU/ml). All samples were measured in triplicate and are shown as mean (SEM) (n = 30). PHA was used at a concentration of 10 μg/ml and 5 μg/ml for PBMC and LPL, respectively. OKT3 was used at a dilution of 1:50 and β-galactosidase was 1 μg/ml. | ||||||
| PHA | 20574 (3974) | 25645 (4735) | 0.14 | 8378 (2086) | 7360 (1744) | 0.34 |
| OKT3 | 10112 (2318) | 12556 (2166) | 0.31 | 5475 (666) | 4745 (1043) | 0.56 |
| β-gal | 651 (110) | 670 (124) | 0.39 | 601 (165) | 605 (173) | 0.96 |