Multiple mRNA expression of nuclear factor κB dependent genes in biopsies of colonic mucosa from patients with collagenous colitis, active ulcerative colitis, or uninflamed bowel
| Gene name | Uninflamed bowel (n = 7) | Collagenous colitis (n = 8) | Ulcerative colitis (n = 6) | |||
|---|---|---|---|---|---|---|
| Median | Range | Median | Range | Median | Range | |
| iNOS, inducible nitric oxide synthase; IL, interleukin; IFN, interferon; IκB, inhibitor of nuclear factor κB. | ||||||
| Expression was analysed by a microarray system and quantified by densitometry. | ||||||
| Data were normalised by dividing individual densitometric values with the mean value of all housekeeping genes provided with the array. The table shows mRNA expression from normalised data for each gene. | ||||||
| p<0.05 by one way ANOVA or Kruskal-Wallis test (see statistics) compared with *uninflamed bowel or †collagenous colitis. | ||||||
| iNOS | 5 | (1–11) | 24.5* | (11–115) | 54.5* | (31–258) |
| Complement factor B | 26 | (23–112) | 91.5* | (62–145) | 197.5*† | (102–589) |
| IL-1α | 8 | (2–18) | 19* | (11–35) | 19* | (9–48) |
| IFN regulating factor 1 | 42 | (29–178) | 111.5* | (74–170) | 71 | (57–188) |
| IFN-β | 5 | (1–11) | 12.5* | (4–26) | 9 | (7–51) |
| IL-1 receptor associated kinase 2 | 6 | (4–51) | 4* | (1–9) | 11.5† | (4–16) |
| Serum amyloid A1 | 14 | (6–23) | 23.5 | (5–104) | 134.5*† | (68–1038) |
| Complement C3 precursor | 48 | (24–196) | 74 | (20–251) | 225.5* | (58–751) |
| IL-8 | 6 | (2–12) | 14.5 | (5–288) | 26* | (14–150) |
| Toll-like receptor 5 | 5 | (3–20) | 8 | (6–12) | 15*† | (14–44) |
| Angiotensinogen | 10 | (6–18) | 14 | (8–28) | 24.5* | (9–65) |
| IL-1 receptor type 1 | 49 | (26–51) | 43.5 | (25–67) | 69*† | (38–115) |
| IκB kinase alpha | 48 | (36–55) | 36 | (23–59) | 29.5* | (14–42) |