Cell yield and viability of magnetically isolated and cultured primitive progenitor cells from fetal livers in the first trimester
| Embryonic age of FL (weeks) | Total No of cells isolated (×106) | No of CD117+/CD34+/Lin−cells obtained (×104) | Viability (%) | No of cell subpassages obtained | Approximate No of cells obtained at indicated passage |
|---|---|---|---|---|---|
| *Cells still in culture at present. | |||||
| FL, fetal liver. | |||||
| 6 | 6 | 3.1 | 93 | 4 | ∼5×105 |
| 6 | 5.6 | 2 | 95 | 4 | ∼4.5×105 |
| 6.5 | 10 | 4.5 | 94 | 2 | ∼8×105 |
| 6.5 | 6.8 | 2.5 | 97 | 2 | ∼10×105 |
| 8 | 8 | 3.2 | 92 | 2 | ∼7×104 |
| 8.5 | 15 | 6.5 | 90 | 12 | ∼550×106 |
| 8 | 7 | 3.2 | 95 | 12 | ∼400×106 |
| 8.5 | 18 | 6.5 | 92 | 10 | ∼70×106 |
| 9.5 | 13 | 5.5 | 97 | 9* | ∼50×106 |
| 9 | 11 | 7.5 | 94 | 12* | ∼307×106 |
| 9 | 5.6 | 4.5 | 93 | 12 | ∼360×106 |
| 9.5 | 10.5 | 7.5 | 92 | 12* | ∼320×106 |
| 9.5 | 17.6 | 8.8 | 95 | 12* | ∼360×106 |
| 9 | 14 | 13.3 | 96 | 11 | ∼272×106 |
| 9.5 | 15.5 | 9.5 | 97 | 14* | ∼389×106 |
| 9.5 | 13.6 | 12 | 91 | 11 | ∼245×106 |