Anti-ED1mouse monoAb (BMA) | 1:500 |
Alexa Fluor 488 conjugated anti-mouse IgG donkey polyAb (Invitrogen) | 1:500 |
Alexa Fluor 568 conjugated anti-mouse IgG donkey polyAb (Invitrogen) | 1:500 |
Anti-ED2 mouse monoAb (BMA) | 1:500 |
Alexa Fluor 488 conjugated anti-mouse IgG donkey polyAb (Invitrogen) | 1:500 |
Anti-PGP9.5 rabbit polyAb (UltraClone) | 1:3000 |
Alexa Fluor 568 conjugated anti-rabbit IgG goat polyAb (Invitrogen) | 1:500 |
Anti-α7nAChR(C-20) goat polyAb (Santa Cruz Biotechnology) | 1:200 |
Alexa Fluor 488 conjugated anti-goat IgG rabbit polyAb (Invitrogen) | 1:500 |
FITC-conjugated α-bungarotoxin (Biotium, Hayward) | 5 μg/ml |