Multivariate-adjusted HRs (95% CI) for a metachronous gastric cancer according to DNA methylation levels of the three genes
| Quartile of DNA methylation level | p for trend | ||||
|---|---|---|---|---|---|
| Variable | Q1 (lowest) | Q2 | Q3 | Q4 (highest) | |
| No. of patients | 195 | 196 | 196 | 195 | |
| Authentic metachronous gastric cancers | |||||
| miR-124a-3 | |||||
| No. of events | 12 | 17 | 15 | 22 | |
| HR* (95% CI) | 1 | 1.33 (0.60 to 2.93) | 1.15 (0.51 to 2.61) | 2.30 (1.03 to 5.10) | 0.057 |
| p Value | 0.49 | 0.73 | 0.042 | ||
| EMX1 | |||||
| No. of events | 12 | 17 | 16 | 21 | |
| HR* (95% CI) | 1 | 1.56 (0.71 to 3.41) | 1.21 (0.55 to 2.67) | 1.88 (0.86 to 4.13) | 0.20 |
| p Value | 0.27 | 0.64 | 0.11 | ||
| NKX6-1 | |||||
| No. of events | 14 | 11 | 20 | 21 | |
| HR* (95% CI) | 1 | 0.76 (0.33 to 1.74) | 1.39 (0.66 to 2.96) | 1.52 (0.72 to 3.21) | 0.12 |
| p Value | 0.52 | 0.39 | 0.27 | ||
| All metachronous gastric cancers | |||||
| miR-124a-3 | |||||
| No. of events | 16 | 20 | 21 | 24 | |
| HR* (95% CI) | 1 | 1.30 (0.64 to 2.64) | 1.31 (0.65 to 2.64) | 1.99 (0.97 to 4.09) | 0.072 |
| p Value | 0.47 | 0.45 | 0.061 | ||
| EMX1 | |||||
| No. of events | 16 | 20 | 19 | 26 | |
| HR* (95% CI) | 1 | 1.47 (0.74 to 2.95) | 1.16 (0.58 to 2.34) | 1.84 (0.92 to 3.67) | 0.150 |
| p Value | 0.27 | 0.68 | 0.082 | ||
| NKX6-1 | |||||
| No. of events | 18 | 16 | 24 | 23 | |
| HR* (95% CI) | 1 | 0.93 (0.46 to 1.87) | 1.41 (0.72 to 2.76) | 1.42 (0.71 to 2.81) | 0.19 |
| p Value | 0.83 | 0.32 | 0.32 | ||
p values <0.05 are shown in bold type.
*Adjusted for hospital, gender and age (<50, 50–59, 60–69 or ≥70), H. pylori infection before the enrolment (positive or negative), pepsinogen index, past history of endoscopic resection (0, 1, 2 or 3 times), pack-years of smoking (0, 1–39 or ≥40), and green vegetable intake (≤2 days per week, 3–4 days per week, or almost daily).