Genomic instability and tumour growth for YAMC clones and control cell lines
| Treatment, cell lines and subclones | Number of treatments* | Cells with aneuploidy and tetraploidy (%±SD)† | Tumour size (mm3) | IHC staining for keratins‡ | Histopathology of tumours |
|---|---|---|---|---|---|
| Untreated | |||||
| HCT116 | None | ND | 1751 | + | Poorly differentiated carcinoma |
| YAMC | 10 | 10.6±3.5 | – | ||
| Treated with 4-hydroxy-2-nonenal | |||||
| H1 | 10 | 23.8 | – | ||
| H2 | 10 | 24.2 | 61 | + | Poorly differentiated carcinoma |
| H3 | 10 | 13.2, 11.8 | 30 | − | Acute/chronic inflammation |
| H4 | 10 | 30.5±4.7 | – | ||
| H5 | 10 | 16.1±4.9 | 201 | + | Poorly differentiated carcinoma |
| H6 | 10 | 22.6 | – | ||
| H7 | 10 | 33.8±9.2 | 195 | + | Poorly differentiated carcinoma |
| H8 | 10 | 18.9, 24.4 | – | ||
| H8-1 | 8 | 40.7±13.9 | – | ||
| H8-4 | 8 | 90.8 | – | ||
| H9 | 10 | 26.9, 16.6 | – | ||
| Exposed to Enterococcus faecalis-infected macrophages | |||||
| 37M10-1 | 10 | 17.5±8.7 | – | ||
| 37M10-3 | 10 | 22.8, 38.0 | 54 | − | Lymphoid mass |
| 37M10-5 | 10 | 77.0±13.5 | – | ||
| 37M10-6 | 10 | 31.1±13.3 | – | ||
| 37M10-7 | 10 | 77.3 | – | ||
| M3 | 10 | 8.4 | 62 | + | Poorly differentiated carcinoma |
| M11 | 10 | ND | 584 | + | Poorly differentiated carcinoma invading skin |
| M12 | 10 | ND | – | ||
| M13 | 10 | ND | 661 | + | Poorly differentiated carcinoma |
| M14 | 10 | 84.9±19.3 | – | ||
| M15 | 10 | 32.3±8.4 | 1117 | + | Poorly differentiated carcinoma invading skin |
| M16 | 10 | 21.3 | – | ||
| M17 | 10 | 30.1 | 74 | + | Poorly differentiated carcinoma invading muscle |
| M6-1 | 6 | 33.4 | – | ||
*YAMC cells were fed twice a week and subcultured once a week for 10 successive weeks.
†Average and SD calculated for experiments repeated ≥3 times. For experiments with <3 repeats, percentages listed for each individual experiment.
‡See materials and methods for immunohistochemical (IHC) staining using a pan-keratin reagent.
ND, not determined; +, positive staining in cancer cell cytoplasm; –, negative staining.