Gene expression of neuronal and inducible NOS
| Age (days) | Gene | Control | BB-DP | p Value | BB-DPH | p Value |
|---|---|---|---|---|---|---|
| 70 | nNOS-α a | 1±0.39 | 1.85±1.35 | P=0.4 | ||
| nNOS-α b | 1±0.31 | 2.10±1.11 | P=0.4 | |||
| nNOS-α c | 1±0.12 | 3.74±1.85 | P=0.3 | |||
| nNOS-β | 1±0.36 | 1±0.78 | P=0.8 | |||
| iNOS | 1±0.12 | 1.18±0.34 | P=0.9 | |||
| 220 | nNOS-α a | 1±0.12 | 0.73±0.37 | P=0.6 | 0.86±0.13 | P=0.4 |
| nNOS-α b | 1±0.16 | 0.76±0.34 | P=0.8 | 0.55±0.11 | P=0.1 | |
| nNOS-α c | 1±0.25 | 0.87±0.33 | P=0.9 | 1.09±0.23 | P=0.8 | |
| nNOS-β | 1±0.93 | 2.40±2.48 | P=0.1 | 2.17±0.49 | P=0.2 | |
| iNOS | 1±0.23 | 2.5±1.4 | P=0.2 | 1.39±3.5 | P=0.9 |
Gene expression of nNOS splice variants (nNOSα and nNOSβ) and iNOS was measured in control, BB-DP and BB-DPH rats of 70 and 220 days (n=6) by the use of real-time reverse transcriptase PCR. Data were normalised to the housekeeping gene, hypoxanthine phosphoribosyltransferase and expressed as fold change. Data in this table represent the fold change of BB-DP and BB-DPH rats, which is compared with a fold change of one in control rats.
BB-DP, BioBreeding diabetes prone; BB-DPH, hyperglycaemic diabetes-prone BB rats; nNOS, neuronal isoform of nitric oxide synthase; iNOS, inducible isoform of nitric oxide synthase.