Lactate and alanine concentrations and lactate dehydrogenase and alanine aminotransferase activities in tissue extracts
| Tissue | Alanine concentration (μmol/g of wet tissue) | Lactate concentration (μmol/g of wet tissue) | Lactate dehydrogenase activity (mU/mg of protein) (n=3) | Alanine aminotransferase activity (mU/mg of protein) (n=3) |
|---|---|---|---|---|
| Control pancreas (n=19) | 1.82±0.32 | 1.55±0.24 | 204.35±53.1 | 6.10±0.35 |
| Induced pancreatitis (n=12) | 1.19±0.2 | 1.36±0.20 | 300.78±93.0 | 9.09±1.25 |
| 4-month-old pancreas (n=20) | 0.98±0.11 | 1.80±0.20 | 616.44±100.05†(*) | 5.09±1.32 |
| 9-month-old pancreas (n=16) | 1.08±0.30 | 4.24±1.21 | 763.46±106.40†(**) ‡(*) | 3.94±0.23 |
| Tumour (sarcomatoid) (n=13) | 4.58±1.00§(*) ¶(**) | 17.80±4.1 §(***) †(****) ‡(****) | 1008.9±49.25§(*) †(****) ‡(***) | 0.28±0.01 |
| Tumour (PDA) (n=12) | 1.70±0.37 | 8.14±1.67 §(**) †(***) ‡(**) | 1142.44±19.55 §(**) ¶(*) †(****) ‡(****) | 0.17±0.04‡(*) |
Pancreatic tissues from controls (LSL-KrasG12D/+ (n=4); p48Cre (n=4); Pdx-1-Cre (PC) (n=6) and C57BL/6 wild-type (n=5)), 4-month-old and 9-month-old KC mice bearing mPanIN lesions and KPC mice with tumours. Mean±SEM.
*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.
†Significantly different compared with control pancreas.
‡Significantly different compared with induced pancreatitis.
§Significantly different compared with 4-month-old pancreas
¶Significantly different from 9-month-old pancreas.
KC, LSL-KrasG12D/+- p48Cre/+; KPC, LSL-KrasG12/D+;LSL-Tpr53R172H/+;Pdx-1-Cre; n, number of animals; PanIN, pancreatic intraepithelial neoplasia; PDA, pancreatic ductal adenocarcinoma.