Partition of 13C label between alanine and lactate following incubation of [3-13C]pyruvate with pancreatic tissue homogenates and in pancreatic tissues in vivo following intravenous injection of [3-13C]pyruvate
| Pancreatic tissue type | Concentrations of added metabolites | [3-13C]alanine/[3-13C]lactate ratio in pancreatic homogenates | 13C-alanine/13C-lactate ratio in vivo (20 s after intravenous injection of [3-13C]pyruvate) | |
|---|---|---|---|---|
| Alanine (mM) | Lactate (mM) | |||
| Control | 2 | 2 | 3.34 | 2.28 |
| Induced pancreatitis | 1 | 1 | 1.33 | 0.56 |
| 4-month-old mice | 1 | 2 | 0.85 | 0.62 |
| 9-month-old mice | 1 | 4 | 0.11 | 0.17 |
| Tumour (PDA) | 2 | 9 | 0.04 | 0.09 |
Partitioning of the 13C label between alanine and lactate was measured in pancreatic tissue homogenates using dynamic 1H NMR measurements and by 13C NMR in pancreatic tissue that was rapidly excised 20 s after intravenous injection of 0.3 mL of 82 mM [3-13C]pyruvate.
PDA, pancreatic ductal adenocarcinoma.