Table 2

Sequences of PCR primers and their Tm values and product sizes

GeneSequences (S: sense, As: antisense)Tm (°C)Product size (bp)
GAPDH

  • S: 5′-TCCCTCAAGATTGTCAGCAA-3′

  • As: 5′-AGATCCACAACGGATACA TT-3′

58308
IL-1β

  • S: 5′-TCCATGAGCTTTGTACAAGG-3′

  • As: 5′-GGTGCTGATGACCAGTTGG-3′

52246
IL-6

  • S: 5′-CAAGAGACT TCCAGCCAGTTGC-3′

  • As: 5′-TTGCCGAGTAGACCTCATAGTGAC-3′

48614
TNFα

  • S: 5′-AAATGGGCTCCCTCTCATCA-3′

  • As: 5′-AGCCTTGTCCCTTGAAGAGA-3′

52248
MCP-1

  • S: 5′-CAACTCTCACTGAAGCCAGA-3′

  • As: 5′- AAATGGATCTACATCTTGCA-3′

54.5600
iNOS

  • S: 5′-AAGGGAGTGTTGTTCCAGGT-3′

  • As: 5′-CCACCAGCTTCTTCAAAGTG -3′

52196
5-HT4R

  • S: 5′-CTTCGGTGCCATTGAGTTG-3′

  • As: 5′-GCATAGGGCTTGTTGACCAT-3′

58354
α7-nAChR

  • S: 5′-ATGGTGGCAAATGCCTAAG-3′

  • As: 5′-CTCGGAAGCCAATGTAGAGC-3′

58204