Sequences of the oligonucleotides used for site-directed mutagenesis, RT-PCR, qPCR and chromatin immunoprecipitation (ChIP)
| Site-directed mutagenesis | 5′ → 3′ |
| Mutation 1* | S: CGATTGTTTAATGTAATAGTTTGGCAAAATGAATTGCTTTCCAS: GGAAAGCAATTCATTTTGCCAAACTATTACATTAAACAATCG |
| Mutation 2* | S: TCTTGTAAACACTCGTTCCTCACGGAAGGCCGCCGAS: CGGCGGCCTTCCGTGAGGAACGAGTGTTTACAAGA |
| RT-PCR | 5′ → 3′ |
| Endogenous CDX2 | F: CCAGGACGAAAGACAAATATCGAR: GACTCTGCTAGACTCCTCAG |
| Exogenous CDX2 | F: CCAGGACGAAAGACAAATATCGAR: CGACAAGCTTGACATTGAAGC |
| GAPDH | F: ACCATCTTCCAGGAGCGAGR: GGATGACCTTGCCCACAG |
| qPCR | 5′ → 3′ |
| Endogenous CDX2 | F: GTGCTAAACCCCACCGTCACR: GACTCTGCTAGACTCCTCAG |
| LI-Cadherin | TaqMan Gene Expression Assay CDH17 Hs00184865_m1 |
| MUC2 | TaqManGene Expression Assay MUC2, Hs03005094_m1 |
| TBP | TaqMan Gene Expression Assay TBP, Hs99999910_m1 |
| human ChIP | 5′ → 3′ |
| pCDX2 region 1 | F: GCATTAGCAGAAATTCTCTTTTCR:GCATGTGGTAGAAGTTAGGCT |
| pCDX2 region 2 | F: CTAACTTCTACCACATGCCCAR: GCATCTCTGACTTCATCTTACA |
| pCDX2 region 3 | F: GAGTTTCTTGACCGCCCTCTTR: CCTCCAATCACAGGTTCAAAGA |
| pCDX2 region 4 | F: CTTTGAACCTGTGATTGGAGGTR: CTCTACGCACAACCCCTCGAA |
| pCDX2 region 5 | F: CGAGGGGTTGTGCGTAGAGTR: ACAGGCTGGCGTGCGGA |
| pSucrase-Isomaltase | F: GGCTGGTAAGGGTGCAATAAR: GCCTGTTCTCTTTGCTATGTTG |
| mouse ChIP | 5′ → 3′ |
| pCdx2 region 1 | F: CAACGGTGGATTCATTCCGR: GGAAGTATTTGTGCTGACACC |
| pCdx2 region 2 | F: GGTGTCAGCACAAATACTTCCR: GTAATTAGTGGATGGCTGGG |
| pCdx2 region 3 | F: CCCAGCCATCCACTAATTACR: GAAAAGACGATTCTACCTCCAG |
| pCdx2 region 4 | F: CGTTTCCAAACCCAGCTTCCR: CCCCCAGAAACACGATTTGC |
| pCdx2 region 5 | F: GCAAATCGTGTTTCTGGGGGR: GCGGCCTTACGTGATTAACG |
| pSucrase-Isomaltase | F: GATAGGCTTGTGAAAGTGCAATR: CCTGTAGTATCTGCTGTGTTG |
↵* Mutated nucleotides are underlined.