Site-directed mutagenesis | 5′ → 3′ |
Mutation 1* | S: CGATTGTTTAATGTAATAGTTTGGCAAAATGAATTGCTTTCCAS: GGAAAGCAATTCATTTTGCCAAACTATTACATTAAACAATCG |
Mutation 2* | S: TCTTGTAAACACTCGTTCCTCACGGAAGGCCGCCGAS: CGGCGGCCTTCCGTGAGGAACGAGTGTTTACAAGA |
RT-PCR | 5′ → 3′ |
Endogenous CDX2 | F: CCAGGACGAAAGACAAATATCGAR: GACTCTGCTAGACTCCTCAG |
Exogenous CDX2 | F: CCAGGACGAAAGACAAATATCGAR: CGACAAGCTTGACATTGAAGC |
GAPDH | F: ACCATCTTCCAGGAGCGAGR: GGATGACCTTGCCCACAG |
qPCR | 5′ → 3′ |
Endogenous CDX2 | F: GTGCTAAACCCCACCGTCACR: GACTCTGCTAGACTCCTCAG |
LI-Cadherin | TaqMan Gene Expression Assay CDH17 Hs00184865_m1 |
MUC2 | TaqManGene Expression Assay MUC2, Hs03005094_m1 |
TBP | TaqMan Gene Expression Assay TBP, Hs99999910_m1 |
human ChIP | 5′ → 3′ |
pCDX2 region 1 | F: GCATTAGCAGAAATTCTCTTTTCR:GCATGTGGTAGAAGTTAGGCT |
pCDX2 region 2 | F: CTAACTTCTACCACATGCCCAR: GCATCTCTGACTTCATCTTACA |
pCDX2 region 3 | F: GAGTTTCTTGACCGCCCTCTTR: CCTCCAATCACAGGTTCAAAGA |
pCDX2 region 4 | F: CTTTGAACCTGTGATTGGAGGTR: CTCTACGCACAACCCCTCGAA |
pCDX2 region 5 | F: CGAGGGGTTGTGCGTAGAGTR: ACAGGCTGGCGTGCGGA |
pSucrase-Isomaltase | F: GGCTGGTAAGGGTGCAATAAR: GCCTGTTCTCTTTGCTATGTTG |
mouse ChIP | 5′ → 3′ |
pCdx2 region 1 | F: CAACGGTGGATTCATTCCGR: GGAAGTATTTGTGCTGACACC |
pCdx2 region 2 | F: GGTGTCAGCACAAATACTTCCR: GTAATTAGTGGATGGCTGGG |
pCdx2 region 3 | F: CCCAGCCATCCACTAATTACR: GAAAAGACGATTCTACCTCCAG |
pCdx2 region 4 | F: CGTTTCCAAACCCAGCTTCCR: CCCCCAGAAACACGATTTGC |
pCdx2 region 5 | F: GCAAATCGTGTTTCTGGGGGR: GCGGCCTTACGTGATTAACG |
pSucrase-Isomaltase | F: GATAGGCTTGTGAAAGTGCAATR: CCTGTAGTATCTGCTGTGTTG |
↵* Mutated nucleotides are underlined.