Morphological and immunohistochemical findings from different intestinal regions of wild-type and Ctnnb1Δex3/+ mice
| Small bowel segment 1 | Small bowel segment 2 | Small bowel segment 3 | Colon | |||||
| WT | Ctnnb1Δex3/+ | WT | Ctnnb1Δex3/+ | WT | Ctnnb1Δex3/+ | WT | Ctnnb1Δex3/+ | |
| Mean cell number/crypt (SEM) | 35.3 (0.44) | Architecture destroyed | 36.9 (0.39) | 72.65 (1.36) | 35.9 (0.4) | 62.9 (1.14) | 47.1 (0.48) | 59.9 (0.7) |
| p Value | NA | <0.0001 | <0.0001 | <0.0001 | ||||
| Mean Paneth cells/crypt-villus cells (per cent (SEM)) | 8.8/97 (9.1% (0.41)) | 11/153 (7.2% (0.45)) | 11.7/88 (13.3% (0.35)) | 16/169 (9.45% (0.22)) | 10.9/70 (15.53% (0.4)) | 11.1/85 (13.1% (0.33)) | NA | NA |
| p Value | <0.0001 | <0.0001 | <0.0001 | NA | ||||
| Mean entero-endocrine cells/crypt-villus cells (per cent (SEM)) | 0.9/95.1 (1% (0.26)) | 1.2/149 (0.9% (0.21)) | 1.1/89.5 (1.2% (0.26)) | 0.8/175.7 (0.5% (0.12)) | 0.8/70.4 (1.1% (0.17)) | 0.5/93.8 (0.5% (0.1)) | 0.9/53 (1.7% (0.31)) | 0.9/72 (1.2% (0.13)) |
| p Value | 0.2 | 0.02 | 0.05 | 0.1 | ||||
| Mean goblet cells/crypt-villus cells (per cent (SEM)) | 7.1/95 (7.5% (0.37)) | 2.1/124.7 (1.7% (0.27)) | 6.1/89.5 (6.9% (0.41)) | 1.9/174.9 (1.1% (0.31)) | 7.4/67.7 (10.9% (0.7)) | 1.2/80 (1.5% (0.4)) | 13.5/54.6 (24.6% (1.9)) | 11.2/70.3 (16% (1.55)) |
| p Value | <0.0001 | <0.0001 | <0.0001 | =0.03 | ||||
| Mean Lgr5+ cells/crypt-villus cells (per cent (SEM)) | 4.1/94 (4.4% (0.4)) | 150/157.8 (99% (1.3)) | 3.4/92.5 (3.7% (0.23)) | 86/170.2 (50.5% (3.4)) | 2.1/69.3 (3% (0.45)) | 34.2/87 (39% (1.23)) | 0.8/50.1 (1.59% (0.3)) | 20.1/64 (31% (1.7)) |
| p Value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | ||||
| Mean Ki-67+ cells/crypt-villus cells (per cent (SEM)) | 19.2/98 (19.8% (1.16)) | 84.5/149 (56.71% (2.3)) | 23/89.7 (25.3% (1.23)) | 83/161 (52.6% (2.99)) | 19.9/66 (30% (1.32)) | 39/80 (48.7% (2.8)) | 14/52 (27% (1.34)) | 27/71 (38% (1.09)) |
| p Value | <0.0001 | <0.0001 | <0.0001 | <0.0001 | ||||
| Mean number cleaved caspase 3+ cells/1000 nuclei (SEM) | 0.6 (0.04) | 26.5 (1.1) | 0.7 (0.1) | 7.3 (1.3) | 0.8 (0.1) | 5.3 (0.7) | 1.1 (0.1) | 5.5 (0.3) |
| p Value | 0.002 | 0.002 | 0.002 | 0.002 | ||||
| Mean crypts in fission/colon (SEM) | NA | NA | NA | NA | NA | NA | 0.25 (0.25) | 26 (4.2) |
| p Value | NA | NA | NA | 0.03 | ||||
| β-Catenin immunostain | Membranous | Heavy nuclear/cytoplasmic | Membranous | Membranous on villi tips and crypt bases. Heavy cytoplasmic elsewhere | Membranous | Cytoplasmic staining in mid-crypt dysplastic areas. Membranous elsewhere | Membranous | Membranous |
The table shows the mean number and percentage of cells per tissue unit on well-orientated cross-sections. A minimum of 50 crypts/crypt-villus units were counted from four different mutant mice. The number of crypts in obvious fission were counted in entire colon rolls from four wild-type and four mutant mice. p Values were calculated using Mann–Whitney U test (Prism, Graphpad Software, La Jolla, California, USA).
WT, wild-type.