Overview lineage tracing results Sanger confirmation
| Gland* | Histology | CCO staining | mtDNA resequencing | TP53 status |
|---|---|---|---|---|
| 1 | Metaplastic columnar epithelium without goblet cells | Deficient | Mutant (16290C>T) | Heterozygous TP53 mutation (p.R273C) |
| 2 | Metaplastic columnar epithelium with goblet cells | Proficient | Wild-type | Wild-type |
| 3 | Metaplastic columnar epithelium without goblet cells | Deficient | Mutant (16290C>T) | Heterozygous TP53 mutation (p.R273C) |
| 4 | Metaplastic columnar epithelium with goblet cells | Proficient | Wild-type | Wild-type |
| 5 | Oesophageal adenocarcinoma | Deficient | Mutant (16290C>T) | Bi-allelic TP53 mutation (p.R273C) |
| 6 | Oesophageal adenocarcinoma | Deficient | Mutant (16290C>T) | Bi-allelic TP53 mutation (p.R273C) |
| NA | Stroma control | NA | Wild-type | Wild-type |
*Refers to glands marked in figure 1B. CCO, cytochrome c oxidase; mtDNA, mitochondrial DNA.