Table 1

Univariate and multivariate-adjusted HRs (95% CI) for a metachronous gastric cancer according to DNA methylation levels of the three genes

	Univariate					Multivariate*
	Quartile of DNA methylation level					Quartile of DNA methylation level
Variable	Q1 (lowest)	Q2	Q3	Q4 (highest)	p for trend	Q1 (lowest)	Q2	Q3	Q4 (highest)	p for trend
No of patients (795)
miR-124a-3	198	199	199	199		198	199	199	199
EMX1	198	199	199	199		198	199	199	199
NKX6-1	198	199	199	199		198	199	199	199
All metachronous gastric cancers
miR-124a-3
No of events	18	33	32	50		18	33	32	50
HR (95% CI)	1	1.73 (0.97 to 3.08)	1.64 (0.91 to 2.94)	2.89 (1.66 to 5.02)	0.0002	1	1.64 (0.90 to 2.99)	1.48 (0.80 to 2.74)	2.57 (1.43 to 4.61)	0.002
EMX1
No of events	18	28	40	47		18	28	40	47
HR (95% CI)	1	1.47 (0.81 to 2.67)	2.09 (1.19 to 3.67)	2.63 (1.51 to 4.56)	0.0001	1	1.46 (0.79 to 2.68)	1.75 (0.98 to 3.13)	2.33 (1.31 to 4.15)	0.0027
NKX6-1
No of events	21	24	37	51		21	24	37	51
HR (95% CI)	1	1.13 (0.63 to 2.05)	1.65 (0.95 to 2.85)	2.42 (1.42 to 4.10)	0.0002	1	1.12 (0.61 to 2.06)	1.61 (0.91 to 2.84)	2.26 (1.30 to 3.92)	0.0008
Authentic metachronous gastric cancers
miR-124a-3
No of events	14	30	26	46		14	30	26	46
HR (95% CI)	1	2.03 (1.07 to 3.85)	1.73 (0.89 to 3.33)	3.53 (1.91 to 6.53)	<0.0001	1	1.93 (1.00 to 3.75)	1.50 (0.75 to 2.97)	3.00 (1.58 to 5.72)	0.0017
EMX1
No of events	15	24	35	42		15	24	35	42
HR (95% CI)	1	1.52 (0.79 to 2.90)	2.21 (1.20 to 4.07)	2.87 (1.58 to 5.23)	0.0001	1	1.44 (0.74 to 2.81)	1.79 (0.95 to 3.38)	2.45 (1.31 to 4.58)	0.0028
NKX6-1
No of events	17	18	33	48		17	18	33	48
HR (95% CI)	1	1.07 (0.55 to 2.09)	1.86 (1.02 to 3.89)	2.92 (1.64 to 5.20)	<0.0001	1	1.03 (0.52 to 2.04)	1.74 (0.93 to 3.24)	2.63 (1.44 to 4.82)	0.0001

*Adjusted for hospital, gender and age (<50, 50–59, 60–69 or ≥70), pepsinogen index, history of endoscopic submucosal dissection (0, 1, 2 or 3 times), pack-years of smoking (0, 1–39 or ≥40) and green vegetable intake (≤2 days/week, 3–4 days/week or almost daily).