Sequences of the mouse primers used for real-time PCR (always in 5′≥3′ orientation)
| Target gene | Forward sequence | Reverse sequence |
|---|---|---|
| Acta2 (αSMA) | GCTGAAGTATCCGATAGAACACG | GGTCTCAAACATAATCTGGGTCA |
| ActRIIB | AGGCAACTTCTGCAACGAG | CTTCCGATGACGATACATCCAG |
| Albumin | GAAGACCCCAGTGAGTGAGC | CAGTCGAGAAGCAGGTGTCC |
| ALK1 | GGCCTTTTGATGCTGTCG | ATGACCCCTGGCAGAATG |
| ALK2 | GTGGAAGATTACAAGCCACCA | GGGTCTGAGAACCATCTGTTAGG |
| BMP-9 | CAGAACTGGGAACAAGCATCC | GCCGCTGAGGTTTAGGCTG |
| β-Actin | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| β2 Microglobulin | TTCTGGTGCTTGTCTCACTGA | CAGTATGTTCGGCTTCCCATTC |
| CD11b | AAACCACAGTCCCGCAGAGA | CGTGTTCACCAGCTGGCTTA |
| Col1a1 | TCAGAGGCGAAGGCAACAGT | CCCCAAGTTCCGGTGTGA |
| Desmin | TACACCTGCGAGATTGATGC | ACATCCAAGGCCATCTTCAC |
| E-cadherin | ACTGTGAAGGGACGGTCAAC | GGAGCAGCAGGATCAGAATC |
| Endoglin | CCCTCTGCCCATTACCCTG | GTAAACGTCACCTCACCCCTT |
| Fibronectin | ATGTGGACCCCTCCTGATAGT | GCCCAGTGATTTCAGCAAAGG |
| Gusb | AAAATGGAGTGCGTGTTGGGTCG | CCACAGTCCGTCCAGCGCCTT |
| Hamp1 | ATACCAATGCAGAAGAGAAGG | AACAGATACCACACTGGGAA |
| Hnf4α | GGCATGGATATGGCCGACTAC | TTCAGATGGGGACGTGTCATT |
| Id1 | CTTCAGGAGGCAAGAGGAAA | CAAACCCTCTACCCACTGGA |
| Lyve-1 | CAGCACACTAGCCTGGTGTTA | CGCCCATGATTCTGCATGTAGA |
| Mki67 | ATCATTGACCGCTCCTTTAGGT | GCTCGCCTTGATGGTTCCT |
| Pcna | TACAGCTTACTCTGCGCTCC | TGTCTGCATTATCTTCAGCCCT |
| Pdgfb | CATCCGCTCCTTTGATGATCTT | GTGCTCGGGTCATGTTCAAGT |
| Peptidylprolyl isomerase A | CAAATGCTGGACCAAACACAAACG | GTTCATGCCTTCTTTCACCTTCCC |
| Pparγ | AGCTGTCATTATTCTCAGTGGAGAC | CTGTCTTCTTGATCACATGCAGTAG |
| Smad6 | AGCATTTTCTACGACCTACCTCA | CCTTGATGGAGTAACCCGGTG |
| Snai1 | CAACTATAGCGAGCTGCAGGA | ACTTGGGGTACCAGGAGAGAGT |
| TGF-β1 | GGTCACCCGCGTGCTAATG | CAGAAGTTGGCATGGTAGCC |
| CTGF | GGGCCTCTTCTGCGATTTC | ATCCAGGCAAGTGCATTGGTA |
| VEGF-A | AACGATGAAGCCCTGGAGTG | AACAAGGCTCACAGTGAACG |
| Vimentin | CGGCTGCGAGAGAAATTGC | CCACTTTCCGTTCAAGGTCAAG |
ALK, activin-like kinase; αSMA, α smooth muscle actin; BMP, bone morphogenetic protein; CTGF, connective tissue growth factor; Pcna, proliferation cell nuclear antigen; Smad, small mothers against decapentaplegic; TGF, transforming growth factor; VEGF, vascular endothelial growth factor.