Regular ArticleQuantification of Adherent and Nonadherent Cells Cultured in 96-Well Plates Using the Supravital Stain Neutral Red
References (0)
Cited by (92)
Cetuximab-oxaliplatin-liposomes for epidermal growth factor receptor targeted chemotherapy of colorectal cancer
2015, Journal of Controlled ReleaseCitation Excerpt :After drug exposure times, cells were rinsed with PBS and new fresh medium was added. Cell viability was measured at 72 h post-treatment using the Neutral Red Assay [27]. Data were expressed as concentration of L-OH that gives a 50% inhibition of cell growth compared to untreated or control cells (IC50).
Application of different methods to formulate PEG-liposomes of oxaliplatin: Evaluation in vitro and in vivo
2012, European Journal of Pharmaceutics and BiopharmaceuticsCitation Excerpt :After 24 h, cells were treated with several concentrations (ranged from 0.1 to 50 μM) of free oxaliplatin, empty liposomes or oxaliplatin liposomes for 72 h. The survival cells after each treatment were measured with the colorimetric Neutral Red Assay [33]. The optical density was read at 540 nm (Labsystems iEMS Reader MF).
Non-glycosylated BMP-2 can induce ectopic bone formation at lower concentrations compared to glycosylated BMP-2
2012, Journal of Controlled ReleaseCitation Excerpt :After 72 h ALP enzymatic activity was quantified by measuring the formation of p-nitrophenol from p-nitrophenyl phosphate (PNPP, Sigma-Aldrich, St. Louis, MO, USA) as described previously [28]. ALP enzymatic activity was corrected for differences in cell number as determined by a Neutral Red assay [29]. Two independent runs were performed, with for each run freshly isolated primary rat bone marrow-derived osteoblast-like cells (OBLCs) following the procedure described by Maniatopoulos et al. [30].
New insights into the structural requirements for pro-apoptotic agents based on 2,4-diaminoquinazoline, 2,4-diaminopyrido[2,3-d]pyrimidine and 2,4-diaminopyrimidine derivatives
2011, European Journal of Medicinal ChemistryCitation Excerpt :In all cases, the concentration of solvent in the culture medium did not exceed 0.5% (v/v). The evaluation of cytotoxic potential and selectivity against human cell lines was determined according to previously reported methods [9,10], by using the neutral red assay as described by Lowik et al. [38] (see Supplementary data for details). Survival percentage was determined at the screening concentrations of 20 and 100 μM, using the survival percentage obtained with the cells treated only with the solvent (DMSO at 0.5%) as a reference.
Osteo-transcriptomics of human mesenchymal stem cells: Accelerated gene expression and osteoblast differentiation induced by vitamin D reveals c-MYC as an enhancer of BMP2-induced osteogenesis
2010, BoneCitation Excerpt :To quantify alkaline phosphatase (ALP) enzymatic activity hMSCs were seeded in 96-well tissue culture plates and cultured for different time intervals under osteogenic differentiation conditions as described above. ALP activity was measured enzymatically as described by Van der Plas et al. [22] and corrected for differences in cell number based on neutral red staining [23]. For histochemical analysis of ALP activity, cells were fixed with 3.7% formaldehyde.
Pharmacodynamics of cisplatin-loaded PLGA nanoparticles administered to tumor-bearing mice
2010, European Journal of Pharmaceutics and Biopharmaceutics