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Hepatocyte Growth Factor Region Specifically Stimulates Gastrointestinal Epithelial Growth in Primary Culture

https://doi.org/10.1006/bbrc.1994.2827Get rights and content

Abstract

Hepatocyte growth factor (HGF) stimulated the growth of fetal rat gastrointestinal epithelial cells in primary culture with a clear dose-response relationship The epithelial response to the mitogenic activity of HGF was different among the region of the gastro-intestinal tract; glandular stomach most responsive, followed by intestine and forestomach. The interaction of HGF with other growth factors in inducing the epithelial growth was also different depending on the type of the epithelial cells, indicating a region-specific growth regulation in the gastro-intestinal tract. Analyses using Northern blot and RT-PCR revealed that HGF mRNA was expressed only in mesenchymes but not in epithelia of the gastro-intestinal tract while expression of c-met (HGF receptor) gene was observed in both tissues. These results suggest that gastro-intestinal mesenchymes secrete HGF which stimulates the growth of attaching epithelial cells by a paracrine mechanism and that the epithelial response to HGF is controlled by a region-specific growth regulatory mechanism.

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    Citation Excerpt :

    HGF was first identified in 1984 as a stimulant for hepatocyte DNA synthesis in rats [48]. However, after further investigation, its expression was detected outside of the liver in several organs, including the small intestine [49, 50]. In cell culture models, HGF was found to stimulate the proliferation and migration of intestinal epithelial cells and to promote tubular cell formation and growth [33].

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