Endothelial Nitric Oxide Synthase Gene Transfer Inhibits Platelet-Derived Growth Factor-BB Stimulated Focal Adhesion Kinase and Paxillin Phosphorylation in Vascular Smooth Muscle Cells

doi:10.1006/bbrc.1997.7035

Biochemical and Biophysical Research Communications

Volume 236, Issue 3, 30 July 1997, Pages 706-711

https://doi.org/10.1006/bbrc.1997.7035 Get rights and content

Abstract

Stimulation of smooth muscle (VSM) cells of guinea pig coronary artery by platelet-derived growth factor (PDGF)-BB retards paxillin mobility (mobility shift) in SDS-PAGE in a time-dependent manner. This mobility shift may be due to tyrosine phosphorylation of paxillin. eNOS gene transfer by replication deficient recombinant adenovirus vector AD5/RSVeNOS in VSM cells inhibited PDGF-BB-stimulated mobility shift and tyrosine phosphorylation of paxillin. Concomitantly, tyrosine phosphorylation of focal adhesion kinase (FAK) was also inhibited. The inhibition of paxillin and FAK tyrosine phosphorylation did not affect stress fiber and focal adhesion formation. Considering the importance of FAK and paxillin in cell migration and proliferation, these results suggest that the FAK-paxillin pathway is a target for NO action to inhibit VSM cell migration and proliferation.

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Regular ArticleEndothelial Nitric Oxide Synthase Gene Transfer Inhibits Platelet-Derived Growth Factor-BB Stimulated Focal Adhesion Kinase and Paxillin Phosphorylation in Vascular Smooth Muscle Cells☆

Abstract

J. Biol. Chem.

Gastroenterology

J. Biol. Chem.

J. Biol. Chem.

Science

BioEssays

J. Biol. Chem.

J. Biol. Chem.

J. Cell Biol.

Am. J. Physiol.

J. Endocrinol.

Mol. Biol. Cell.

J. Cell Biol.

Cell Growth Differentiation

Nature

Nature

J. Cell Sci.

BioEssays

Nature

FASEB J.

J. Clin. Invest.

Regular Article
Endothelial Nitric Oxide Synthase Gene Transfer Inhibits Platelet-Derived Growth Factor-BB Stimulated Focal Adhesion Kinase and Paxillin Phosphorylation in Vascular Smooth Muscle Cells☆