Scavenger Receptors Mediate Adhesion of Activated B Lymphocytes

doi:10.1006/excr.1997.3876

Experimental Cell Research

Volume 239, Issue 1, 25 February 1998, Pages 16-22

https://doi.org/10.1006/excr.1997.3876 Get rights and content

Abstract

The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of SR-A expression by high endothelial cells of venules and on follicular dendritic cells in peripheral lymph nodes prompted us to investigate whether SR-A can act as an addressin for lymphocytes. We describe here that activated B cells adhere to CHO cells transfected with either the type I or type II isoform of SR-A. In contrast, resting B cells isolated from peripheral blood did not adhere to SR-A transfected CHO cells. Other types of leukocytes did not bind to SR-A. The adhesive properties of B lymphocytes in different stages of activation were further explored using lymphoma cell lines of the B cell lineage. Thein vitroEBV-transformed B cell line IARC171 showed enhanced adhesiveness to SR-A, whereas the Burkitt lymphoma cell lines, BL41, Rael, and BL16 did not. The SR-A-dependent adhesion of B-lymphoblasts occurred both at 37 and 4°C, suggesting that it was not dependent on cell metabolism. The known polyanionic ligands for SR-A, fucoidan, and acetylated low density lipoprotein, which bind to a positively charged portion of the collagen-like domain of SR-A, did not inhibit adhesion. This finding suggests that SR-A mediates adhesion of activated B lymphocytes through a binding site that differs from the one that binds polyanionic ligands. Together, our data suggest that SR-A plays a role in the recruitment of activated B cells into lymph nodes and inflammatory lesions by acting as an adhesion molecule for such cells.

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¹: Current address: Dept. of Medicine, Yodogawa Christian Hospital, Osaka, Japan.

²: To whom correspondence and reprint requests should be addressed. Fax: (+46) 8 51776222. E-mail:[email protected].

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Regular ArticleScavenger Receptors Mediate Adhesion of Activated B Lymphocytes

Abstract

J. Biol. Chem.

J. Biol. Chem.

Methods Enzymol.

Lipoprotein metabolism in the macrophage: Implifications for cholesterol deposition in atherosclerosis

Annu. Rev. Biochem.

Purification and characterization of a bovine acetyl low density lipoprotein receptor

Proc. Natl. Acad. Sci. USA

Type I macrophage scavenger receptor contains alpha-helical and collagen-like coiled coils

Nature

Coiled-coil fibrous domains mediate ligand binding by macrophage scavenger receptor type II

Nature

Recognition and plasma clearance of endotoxin by scavenger receptors

Nature

The type I macrophage scavenger receptor binds to Gram-positive bacteria and recognizes lipoteichoic acid

Proc. Natl. Acad. Sci. USA

Beyond cholesterol: Modifications of low-density lipoprotein that increase its atherogenicity

N. Engl. J. Med.

A role for macrophage scavenger receptors in atherosclerosis and susceptibility to infection

Nature

Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor

Nature

Murine macrophage scavenger receptor: In vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs

Eur. J. Immunol.

Human macrophage scavenger receptors: Primary structure, expression, and localization in atherosclerotic lesions

Proc. Natl. Acad. Sci. USA

Regular Article
Scavenger Receptors Mediate Adhesion of Activated B Lymphocytes