Markers of Mast Cell Degranulation

doi:10.1006/meth.1997.0494

Methods

Volume 13, Issue 1, September 1997, Pages 43-52

https://doi.org/10.1006/meth.1997.0494 Get rights and content

Abstract

Mast cells are the primary effector cells of immediate hypersensitivity reactions in humans. Upon mast cell activation both preformed and newly synthesized mediators are secreted. Histamine can be measured by fluorometric assays, radioenzymatic assays, and immunoassays. These methods have been applied to plasma and urine to detect histamine that had been releasedin vivoand to release histaminein vitrofrom basophils and mast cells. Another mast cell constituent is tryptase, which is a more selective marker of mast cells, because negligible amounts are found in basophils. β-Tryptase is stored in secretory granules and is actively released when mast cells degranulate. α-Protryptase remains in the proenzyme form and is constitutively released from mast cells, and consequently its level in serum reflects total numbers of mast cells. α-Protryptase levels are elevated in serum at baseline in subjects with systemic mastocytosis, whereas β-tryptase is elevated in serum from subjects with systemic anaphylaxis. These markers serve as precise clinical indicators of the involvement of mast cells in human disease.

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^☆: E. Middleton, Jr.C. E. ReedE. F. EllisN. F. Adkinson, Jr.J. W. YungingerW. W. Busse, Eds.

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Regular ArticleMarkers of Mast Cell Degranulation☆

Abstract

J. Allergy

Anal. Biochem.

J. Allergy Clin. Immunol.

J. Allergy Clin. Immunol.

Biochemistry

Life Sci.

J. Allergy Clin. Immunol.

J. Allergy Clin. Immunol.

J. Immunol. Methods

J. Immunol. Methods

J. Allergy Clin. Immunol.

J. Immunol. Methods

J. Allergy Clin. Immunol.

J. Chromatogr.

J. Allergy Clin. Immunol.

J. Invest. Dermatol.

J. Allergy Clin. Immunol.

FEBS Lett.

J. Pediatr.

Am. J. Med.

J. Biol. Chem.

Methods Enzymol.

J. Biol. Chem.

Biochem. Pharmacol.

J. Immunol. Methods

J. Immunol. Methods

J. Allergy Clin. Immunol.

Ann. Fr. Anesth. Reanim.

Lancet

Lancet

J. Allergy Clin. Immunol.

J. Allergy Clin. Immunol.

Mayo Clin. Proc.

Prostaglandins

Allergy: Principals and Practice

Am. J. Pathol.

Proc. Natl. Acad. Sci. USA

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Clin. Exp. Allergy

Am. J. Physiol.

J. Pharmacol. Exp. Ther.

J. Pharmacol. Exp. Ther.

Ann. Clin. Biochem.

Agents Actions

Allergy

Science

Regular Article
Markers of Mast Cell Degranulation☆