Abstract
The aim of this study was to clarify the involvement of basic fibroblast growth factor (bFGF) in gastric ulcer healing. For this purpose, light and electron microscopic immunohistochemical studies for bFGF were performed using an experimental gastric ulcer model of mice. Ulceration was induced by the application of acetic anhydride to the serosal surface of the body of the stomach. Stomach tissues were investigated of mice at 5 days and 3 weeks respectively after treatment and also of untreated normal mice. Five days after treatment an ulcer was seen in the stomach of the experimental mice. Immunohistochemistry revealed that bFGF was localized in fibroblasts in the ulcer bed. The growth factor was distributed throughout the cytoplasm excluding organelles involved in the usual secretory system, such as rough endoplasmic reticulum, Golgi apparatus and secretory vacuoles. bFGF was also detected in the nucleus. Three weeks after treatment the surface of the ulcer lesion was completely covered by regenerated epithelium. The stomach tissues were immunohistochemically negative for bFGF both inside and outside the scar region; untreated normal stomach tissues were also negative for bFGF. These results suggest that the growth factor plays important roles in gastric ulcer healing.
References
Baird A, Böhlen P (1990) Fibroblast growth factors. In: Sporn MB, Roberts AB (eds) Peptide growth factors and their receptors I. Springer, Berlin, pp 369–418
Esch F, Baird A, Ling N, Ueno N, Hill F, Denoroy L, Klepper R, Gospodarowicz D, Böhlen P, Guillemin R (1985) Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc Natl Acad Sci USA 82:6507–6511
Folkman J, Szabo S, Stovroff M, McNeil P, Li W, Shing Y (1991) Duodenal ulcer. Discovery of a new mechanism and development of angiogenic therapy that accelerates healing. Ann Surg 214:414–427
Gospodarowicz D (1990) Fibroblast growth factor. Chemical structure and biologic function. Clin Orthop 257:231–248
Gospodarowicz D, Bialecki H, Thakral TK (1979) The angiogenic activity of the fibroblast and epidermal growth factor. Exp Eye Res 28:501–514
Iwata H, Matsuyama A, Okumura N, Yoshida S, Lee Y, Imaizumi K, Shiosaka S (1991) Localization of basic FGF-like immunoreactivity in the hypothalamo-hypophyseal neuroendocrine axis. Brain Res 550:329–332
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Mignatti P, Morimoto T, Rifkin DB (1992) Basic fibroblast growth factor, a protein devoid of secretory signal sequence, is released by cells via a pathway independent of the endoplasmic reticulum-Golgi complex. J Cell Physiol 151:81–93
Moscatelli D, Presta M, Joseph-Silverstein J, Rifkin DB (1986) Both normal and tumor cells produce basic fibroblast growth factor. J Cell Physiol 129:273–276
Ohtani H, Nakamura S, Watanabe Y, Mizoi T, Saku T, Nagura H (1993) Immunocytochemical localization of basic fibroblast growth factor in carcinomas and inflammatory lesions of the human digestive tract. Lab Invest 68:520–527
Okabe S, Roth JLA, Pfeiffer CJ (1971) A method for experimental, penetrating gastric and duodenal ulcers in rats. Observations on normal healing. Dig Dis 16:277–284
Rhodin JAG, Fujita H (1989) Capillary growth in the mesentery of normal young rats. Intravital video and electron microscope analyses. J Submicrosc Cytol Pathol 21:1–34
Root LL, Shipley GD (1991) Human dermal fibroblasts express multiple bFGF and aFGF proteins. In Vitro Cell Dev Biol 27A:815–822
Story MT (1989) Cultured human foreskin fibroblasts produce a factor that stimulates their growth with properties similar to basic fibroblast growth factor. In Vitro Cell Dev Biol 25:402–408
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
Yabu M, Takaoka H, Hashimoto J, Fujita H (1991) Ultramicroscopic aspects of the conversion of fibroblasts to chondrocytes in the mouse dorsal subfascia induced by bone morphogenetic protein(BMP). Arch Histol Cytol 54:95–102
Yabu M, Takaoka K, Hashimoto J, Fujita H (1992) Immunohistochemical, autoradiographic and electron microscopic studies on the transformation of fibroblasts into chondrocytes in the mouse subfascia induced by bone morphogenetic protein. Histochemistry 97:463–468
Yamamoto N, Matsutani S, Yoshitake Y, Nishikawa K (1991) Immunohistochemical localization of basic fibroblast growth factor in A431 human epidermoid carcinoma cells. Histochemistry 96:479–485
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Yabu, M., Shinomura, Y., Minami, T. et al. Immunohistochemical localization of basic fibroblast growth factor in the healing stage of mouse gastric ulcer. Histochemistry 100, 409–413 (1993). https://doi.org/10.1007/BF00267820
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DOI: https://doi.org/10.1007/BF00267820