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Immunohistochemical localization of basic fibroblast growth factor in the healing stage of mouse gastric ulcer

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Abstract

The aim of this study was to clarify the involvement of basic fibroblast growth factor (bFGF) in gastric ulcer healing. For this purpose, light and electron microscopic immunohistochemical studies for bFGF were performed using an experimental gastric ulcer model of mice. Ulceration was induced by the application of acetic anhydride to the serosal surface of the body of the stomach. Stomach tissues were investigated of mice at 5 days and 3 weeks respectively after treatment and also of untreated normal mice. Five days after treatment an ulcer was seen in the stomach of the experimental mice. Immunohistochemistry revealed that bFGF was localized in fibroblasts in the ulcer bed. The growth factor was distributed throughout the cytoplasm excluding organelles involved in the usual secretory system, such as rough endoplasmic reticulum, Golgi apparatus and secretory vacuoles. bFGF was also detected in the nucleus. Three weeks after treatment the surface of the ulcer lesion was completely covered by regenerated epithelium. The stomach tissues were immunohistochemically negative for bFGF both inside and outside the scar region; untreated normal stomach tissues were also negative for bFGF. These results suggest that the growth factor plays important roles in gastric ulcer healing.

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References

  • Baird A, Böhlen P (1990) Fibroblast growth factors. In: Sporn MB, Roberts AB (eds) Peptide growth factors and their receptors I. Springer, Berlin, pp 369–418

    Google Scholar 

  • Esch F, Baird A, Ling N, Ueno N, Hill F, Denoroy L, Klepper R, Gospodarowicz D, Böhlen P, Guillemin R (1985) Primary structure of bovine pituitary basic fibroblast growth factor (FGF) and comparison with the amino-terminal sequence of bovine brain acidic FGF. Proc Natl Acad Sci USA 82:6507–6511

    Google Scholar 

  • Folkman J, Szabo S, Stovroff M, McNeil P, Li W, Shing Y (1991) Duodenal ulcer. Discovery of a new mechanism and development of angiogenic therapy that accelerates healing. Ann Surg 214:414–427

    Google Scholar 

  • Gospodarowicz D (1990) Fibroblast growth factor. Chemical structure and biologic function. Clin Orthop 257:231–248

    Google Scholar 

  • Gospodarowicz D, Bialecki H, Thakral TK (1979) The angiogenic activity of the fibroblast and epidermal growth factor. Exp Eye Res 28:501–514

    Google Scholar 

  • Iwata H, Matsuyama A, Okumura N, Yoshida S, Lee Y, Imaizumi K, Shiosaka S (1991) Localization of basic FGF-like immunoreactivity in the hypothalamo-hypophyseal neuroendocrine axis. Brain Res 550:329–332

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Google Scholar 

  • Mignatti P, Morimoto T, Rifkin DB (1992) Basic fibroblast growth factor, a protein devoid of secretory signal sequence, is released by cells via a pathway independent of the endoplasmic reticulum-Golgi complex. J Cell Physiol 151:81–93

    Google Scholar 

  • Moscatelli D, Presta M, Joseph-Silverstein J, Rifkin DB (1986) Both normal and tumor cells produce basic fibroblast growth factor. J Cell Physiol 129:273–276

    Google Scholar 

  • Ohtani H, Nakamura S, Watanabe Y, Mizoi T, Saku T, Nagura H (1993) Immunocytochemical localization of basic fibroblast growth factor in carcinomas and inflammatory lesions of the human digestive tract. Lab Invest 68:520–527

    Google Scholar 

  • Okabe S, Roth JLA, Pfeiffer CJ (1971) A method for experimental, penetrating gastric and duodenal ulcers in rats. Observations on normal healing. Dig Dis 16:277–284

    Google Scholar 

  • Rhodin JAG, Fujita H (1989) Capillary growth in the mesentery of normal young rats. Intravital video and electron microscope analyses. J Submicrosc Cytol Pathol 21:1–34

    Google Scholar 

  • Root LL, Shipley GD (1991) Human dermal fibroblasts express multiple bFGF and aFGF proteins. In Vitro Cell Dev Biol 27A:815–822

    Google Scholar 

  • Story MT (1989) Cultured human foreskin fibroblasts produce a factor that stimulates their growth with properties similar to basic fibroblast growth factor. In Vitro Cell Dev Biol 25:402–408

    Google Scholar 

  • Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354

    Google Scholar 

  • Yabu M, Takaoka H, Hashimoto J, Fujita H (1991) Ultramicroscopic aspects of the conversion of fibroblasts to chondrocytes in the mouse dorsal subfascia induced by bone morphogenetic protein(BMP). Arch Histol Cytol 54:95–102

    Google Scholar 

  • Yabu M, Takaoka K, Hashimoto J, Fujita H (1992) Immunohistochemical, autoradiographic and electron microscopic studies on the transformation of fibroblasts into chondrocytes in the mouse subfascia induced by bone morphogenetic protein. Histochemistry 97:463–468

    Google Scholar 

  • Yamamoto N, Matsutani S, Yoshitake Y, Nishikawa K (1991) Immunohistochemical localization of basic fibroblast growth factor in A431 human epidermoid carcinoma cells. Histochemistry 96:479–485

    Google Scholar 

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Yabu, M., Shinomura, Y., Minami, T. et al. Immunohistochemical localization of basic fibroblast growth factor in the healing stage of mouse gastric ulcer. Histochemistry 100, 409–413 (1993). https://doi.org/10.1007/BF00267820

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  • DOI: https://doi.org/10.1007/BF00267820

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