Summary
The BCEI gene codes for a small secreted protein and is expressed in the human mammary tumour cell line MCF7 under oestrogen control and in some breast cancers. We have mapped the gene to chromosome 21 using a panel of somatic hybrid lines, and in situ hybridization has allowed a precise assignment to band 21q223. Two restriction fragment length polymorphisms (RFLP) are described that should be of use in linkage or population studies to test a possible involvement of the BCEI gene in genetic predisposition to breast cancer. This gene should also be a useful marker for the genetic and physical mapping of chromosome 21, and for a better definition of the region involved in the clinical phenotype of Downs syndrome.
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Alwine JC, Kemp DJ, Parker BA, Reiser J, Renart J, Start GR, Wahl GM (1979) Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol 68:220–242
Anderson DE, Ferrell RE, Williams WR (1985) A linkage study of human breast cancer. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:568
Berger R, Bloomfield CD, Sutherland GR (1985) Report of the committee on chromosome rearrangements in neoplasia and on fragile sites. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:490–535
Broca PP (1869) Traité des tumeurs, vols 1, 2. Asselin, Paris
Brown AMC, Jeltsch JM, Roberts M, Chambon P (1984) Activation of pS2 gene transcription is a primary response to estrogen in the human breast cancer cell line MCF-7. Proc Natl Acad Sci USA 81:6344–6348
Camarga M, Cervenka J (1982) Patterns of DNA replication of human chromosomes II: replication map and replication model. Am J Hum Genet 34:757–780
Cohen-Haguenauer O, Van Cong N, Prud'homme JF, Jegou-Foubert C, Gross MS, De Tand MF, Milgrom E, Frézal J (1985) A gene expressed in human breast cancer and regulated by estrogen in MCF-7 cells is located on chromosome 21. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:606
Delabar JM, Goldgaber D, Lamour Y, Nicole A, Huret JL, Grouchy J de, Brown P, Gajdusek DC, Sinet PM (1987) β-amyloid gene duplication in Alzheimer's disease and karyotypically normal Down syndrome. Science 235:1390–1392
Gairard B, Krust A, Rio MC, Koehl C, Bellocq JP, Chambon P, Renaud R (1986) Endocrinology of the breast: basic and clinical aspects. Ann NY Acad Sci 464:443–447
Huret JL, Delabar JM, Marlhens F, Aurias A, Nicole A, Berthier M, Tanzer J, Sinet PM (1987) Down syndrome with duplication of a region of chromosome 21 containing the CuZn superoxide dismutase gene without detectable karyotyping abnormality. Hum Genet 75:251–257
Jakowlew SB, Breathnach R, Jeltsch JM, Masiakowski P, Chambon P (1984) Sequence of the pS2 mRNA induced by estrogen in the human breast cancer cell line MCF-7. Nucleic Acids Res 12:2861–2878
Jeltsch JM, Roberts M, Schatz C, Garnier JM, Brown AMC, Chambon P (1987) Structure of the human oestrogen-responsive gene pS2. Nucleic Acids Res 15:1401–1414
Kang J, Lemaire HG, Unterbeck A, Salbaum JM, Masters CL, Grzeschik KH, Multhaup G, Beyreuther K, Müller-Hill B (1987) The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptor. Nature 325:733–736
King MC, Go RCP, Lynch HT, Elston RC, Terasaki PI, Petrakis NL, Rodgers GC, Lattanzio D, Bailey-Wilson J (1983) Genetic epidemiology of breast cancer and associated cancers in high-risk families. II. Linkage analysis. JNCI 71:463–467
Lötscher E, Grezeschilk KH, Bauer HG, Pohlenz HD, Straubinger B, Zachau HG (1986) Dispersed human immunoglobulin κ lightchain genes. Nature 320:456–458
Lynch HT, Albano WA, Heieck JJ, Mulcahy GM, Lynch JF, Layton MA, Danes BS (1984) Genetics, biomarkers, and control of breast cancer: a review. Cancer Genet Cytogenet 13:43–92
Masiakowski P, Breathnach R, Bloch J, Gannon F, Krust A, Chambon P (1982) Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res 10:7895–7903
Mattei JF, Mattei MG, Baeteman MA, Giraud F (1981) Trisomy 21 for the region 21q223: identification by high-resolution R-banding patterns. Hum Genet 56:409–411
Mattei MG, Philip N, Passage E, Moisan JP, Mandel JL, Mattei JF (1985) DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome. Hum Genet 69:268–271
Moisan JP, Mattei MG, Baeteman-Volkel MA, Mattei JF, Brown AMC, Garnier JM, Jeltsch JM, Masiakowsky P, Roberts M, Mandel J-L (1985) A gene expressed in human mammary tumor cells under estrogen control (BCEI) is located in 21q223 and defines an RFLP. (8th International Workshop on Human Gene Mapping) Cytogenet Cell Genet 40:701–702
Nunez AM, Jakowlev S, Briand JP, Gaire M, Krust A, Rio MC, Chambon P (1987) Characterization of the oestrogen-induced pS2 protein secreted by the human breast cancer cell line MCF-7. Endocrinology 121:1759–1765
Oberlé I, Camerino G, Kloepfer C, Moisan JP Grzeschik KH, Hellkuhl B, Hors-Cayla MC, Van Cong N, Mandel J-L (1986) Characterization of a set of X-linked sequences and of a panel of somatic cell hybrids useful for the regional mapping of the human X chromosome. Hum Genet 72:43–49
Petrakis NL, Ernster VL, King MC (1982) Epidemiology of breast cancer. In: Schottenfeld D, Fraumenl JF (eds) Cancer epidemiology and prevention. Saunders, Philadelphia, pp 800–870
Rodgers CS, Hill SM, Hulten MA (1984) Cytogenetic analysis in human breast carcinoma. I. Nine cases in the diploid range investigated using direct preparations. Cancer Genet Cytogenet 13:95–119
Skolnick MH, Thompson EA, Bishop DT, Cannon LA (1984) Sensitivity of lod scores to a single recombinant observation: linkage of a breast cancer gene to the AB0 locus. Am J Hum Genet 36: 181s
Zabel BU, Naylor SL, Sakaguchi AY, Bell GI, Shows TB (1983) High-resolution chromosomal localization of human genes for amylase, propiomelanocortin, and a DNA fragment (D3S1) by in situ hybridization. Proc Natl Acad Sci USA 80:6932–6936
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Moisan, JP., Mattei, MG. & Mandel, JL. Chromosome localization and polymorphism of an oestrogen-inducible gene specifically expressed in some breast cancers. Hum Genet 79, 168–171 (1988). https://doi.org/10.1007/BF00280558
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DOI: https://doi.org/10.1007/BF00280558