Summary
Antisera specific for three different regions of pancreatic proglucagon were used to examine the distribution of such immunoreactivity in rat hypothalamus. Neurons in the supraoptic and paraventricular nuclei were immunoreactive with an antiserum against glucagon, but not with antisera directed towards the aminoterminal region of proglucagon (glicentin) or the glucagon-like peptide I sequence in the carboxyl-terminal region of proglucagon. These findings confirm a previous report of glucagon-like immunoreactivity in the supraoptic and paraventricular nuclei, but indicate that, while this material is immunochemically related to glucagon, it is not derived from a proglucagon-like precursor.
Similar content being viewed by others
References
Bell GI, Santerre RF, Mullenbach GT (1983) Hamster preproglucagon contains the sequence of glucagon and two related peptides. Nature 302:716–718
Conlon JM, Samson WK, Dobbs RE, Orci L, Unger RH (1979) Glucagon-like polypeptides in canine brain. Diabetes 28:700–702
Han VKM, Hynes MA, Jin C, Towle AC, Lauder JM, Lund PK (1986) Cellular localization of proglucagon/glucagon-like peptide I messenger RNAs in rat brain. J Neurosci Res 16:97–107
Hase T, Kimura H (1985) Distribution of neuronal cell bodies and axons containing glicentin-like immunoreactivity in the rat brain. Acta Histochem Cytochem 18:581–588
Heinrich G, Gros P, Lund PK, Bentley RC, Habener JF (1984) Pre-proglucagon messenger ribonucleic acid: nucleotide and encoded amino acid sequences of the rat pancreatic complementary deoxyribonucleic acid. Endocrinology 115:2176–2181
Inokuchi A, Tomida Y, Yanaihara C, Yui R, Ooomura Y, Kimura H, Hase T, Matsumoto T, Yanaihara N (1986) Glucagon-related peptides in the rat hypothalamus. Cell Tissue Res 246:71–75
Jin S-LC, Han VKM, Simmons JG, Towle AC, Lauder JM, Lund PK (1988) Distribution of glucagonlike peptide I (GLP-I), glucagon, and glicentin in the rat brain: an immunocytochemical study. J Comp Neurol 271:519–532
Larsson L-I, Moody AJ (1980) Glicentin and gastric inhibitory peptide immunoreactivity in endocrine cells of the gut and pancreas. J Histochem Cytochem 28:925–933
Lopez LC, Frazier ML, Su C-J, Kumar A, Saunders GF (1983) Mammalian pancreatic preproglucagon contains three glucagon-related peptides. Proc Natl Acad Sci USA 80:5485–5489
Loren I, Alumets J, Hakanson R, Sundler F, Thorell J (1979) Gut-type glucagon immunoreactivity in nerves of the rat brain. Histochemistry 61:335–341
Lund PK, Simmons JG, Jin C, Han VKM, Towle A, Lauder JM (1984) Localization of glucagon and glucagon-like peptide I in A cells of rat pancreatic islets. Dig Dis Sci 29: [Suppl 8] S50
Majsov S, Heinrich G, Wilson IB, Ravazzola M, Orci L, Habner JF (1986) Preproglucagon gene expression in pancreas and intestine diversifies at the level of post-translational processing. J Biol Chem 261:11880–11889
Moody A, Holst JJ, Thim L, Lindkaer-Jensen S (1981) Relationship of glicentin to proglucagon and glucagon in the porcine pancreas. Nature 289:514–516
Paxinos G, Watson C (1986) The rat brain in stereotaxic coordinates, 2nd edn. Academic Press, London
Sasaki H, Ebitani I, Tominaga M, Yamatani K, Yawata Y, Hara M (1980) Glucagon-like substance in the canine brain. Endocrinol Jpn 1:135–140
Shimizu I, Hirota M, Ohboshi C, Shima K (1987) Identification and localization of glucagon-like peptide-I and its receptor in rat brain. Endocrinology 121:1076–1082
Sofroniew MV, Eckenstein F, Schrell U, Cuello AC (1984) Evidence for colocalization of neuroactive substances in hypothalamic neurons. In: Chan-Palay V, Palay SL (eds) Coexistence of neuroactive substances. Wiley, New York, pp 73–90
Tager H, Hohenboken M, Markese J, Dinerstein RJ (1980) Identification and localization of glucagon-related peptides in rat brain. Proc Natl Acad Sci USA 77:6229–6233
Thim L, Moody AJ (1981) The primary structure of porcine glicentin (proglucagon). Regul Peptide 2:139–150
Tominaga M, Ebitani I, Marubashi S, Kamimura T, Katagiri T, Sasaki H (1981) Species difference of glucagon-like materials in the brain. Life Sci 29:1577–1581
Tominaga M, Kaneda H, Marbuashi S, Kamimura T, Katagiri T, Sasaki H (1984) Synaptosomal localization and release of glucagon-like materials in the rat brain. Brain Res Bull 12:373–375
Triepel J, Elger KH, Forssmann WG (1982) Glicentin-immunoreactive perikarya and varicosities in the guinea pig central nervous system. Neurosci Lett 30:285–289
Triepel J, Elger KH, Mader J, Weindl A, Metz J, Forssmann WG (1984) Neurons of A1/A2 region in the guinea pig medulla oblongata containing glucagon, glicentin, and dopamin-betahydroxylase immunoreactivity. Anat Embryol 170:239–245
Vaillant C, Lund PK (1986) Distribution of glucagon-like peptide I in canine and feline pancreas and gastrointestinal tract. J Histochem Cytochem 34:1117–1121
Vaillant C, Taylor IL (1981) Demonstration of carboxyl-terminal PP-like peptides in endocrine cells and nerves. Peptides 2:31–35
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Salazar, I., Vaillant, C. Glucagon-like immunoreactivity in hypothalamic neurons of the rat. Cell Tissue Res 261, 355–358 (1990). https://doi.org/10.1007/BF00318677
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00318677