Summary
By a cryosurgical method a mucosal defect was produced in the body of the rat stomach, and repair of the gastric ulcer was studied with 3H-thymidine autoradiography. From 18 to 24 hours after cryoinjury, cell proliferation in the fundic mucosa was much increased, as indicated by an increase in the labeling index of the cells in the proliferating cell zone of the mucosa, or by reactive incorporation of 3H-thymidine into the mucous neck, chief and parietal cells. Spatially, the increased cell proliferation was found in a region of the mucosa more than 600 μrn distant from the injury, and lasted for more than 14 days. The mucous neck and chief cells around the ulcer which had incorporated 3H-thymidine seemed to transform into flat cells after mitotic division and then continued to divide. After 3–5 days, so called regenerating epithelium or covering epithelium appeared around the ulcer. The upper part of this regenerating epithelium consists of tall columnar cells, the lower part is composed of cystic glandular structures, in which many 3H-thymidine incorporating cells were seen. The formation of new glands in the mucosal defect appeared to take place by budding from these cystic glandular structures with subsequent differentiation of surface epithelial cells. After 3 weeks the mucosal defect was covered by mucinous glandular structures similar to the proper pyloric mucosa. The proliferating cells were confined to the middle level in the regenerated mucosa.
Similar content being viewed by others
References
Bloom W, Fawcett DW (1968) A textbook of histology, 9th ed. W.B. Saunders, Philadelphia, pp 549–559
Bodic Le L, Bodic Le MF, Sudry P, Lemort J, Joubert B (1979) Etude des effets hémostatiques du laser Argon sur la paroi gastrique du chien. Ann Anat Pathol 24:15–28
Borchard F, Mittelstaedt A, Stux G (1979) Dysplasie im Resektionsmagen und Klassifikationsprobleme verschiedener Dysplasieformen Verh. Dtsch. Ges. Pathol 63:250–257
Breining H, Helpap B, Langer S, Lymberopoulos S (1974) Die Kryonekrose und ihre Abheilung an Magen und Darm. Z Gastroenterol 12:385–394
Finchk ES, Milton GW (1960) Regeneration of gastric mucosa from differentiated cells. J Pathol Bact 80:143–145
Frühmorgen P, Reichenbach HD, Bodem F, Kaduk B, Demling L (1974) Experimental examination on laser endoscopy. Endoscopy 6:116–122
Hattori T, Fujita S (1974) Fractographic study on the growth and multiplication of the gastric gland of the hamster. Cell Tissue Res 153:145–149
Hattori T, Fujita S (1976a) Tritiated thymidine autoradiographic study on cell migration in the gastric gland of the golden hamster. Cell Tissue Res 172:171–184
Hattori T, Fujita S (1976b) Tritiated thymidine autoradiographic study on cell migration and renewal in the pyloric mucosa of golden hamster. Cell Tissue Res 175:49–57
Helpap B (1980) Der kryochirurgische Eingriff und seine Folgen. Morphologische und zellkinetische Analyse. Thieme, Stuttgart-New York
Helpap B, Breining H, Minderjahn A, Lymberopoulos S (1974) Wound healing of Kidney after cryonecrosis. Virchows Arch A Path Anat Histol 363:123–133
Helpap B, Breining H, Lymberopoulos S (1973) Autoradiographic, histological and histochemical investigations on liver of rats following local freezing. Beitr Pathol 148:165–179
Helpap B, Grouls V (1979) Tissue reparation of the liver after thermo- and cryosurgical lesions: comparative cell analytical investigation. Cryobiology 16:473–480
Helpap B, Breining H, Cappel S, Sturm KW, Lymberopoulos S (1976) Wound healing of the brain of rats after cryonecrosis. Autoradiographic investigations with 3H-thymidine. Virchows Arch B Cell Path 22:151–161
Helpap B, Yamashita K, Breining H, Lymberopoulos S (1974) Autoradiographische Untersuchungen mit 3H-Thymidin an der Rattenmilz nach Kryonekrose. Experientia 30:273–275
Hunt TE (1958) Regeneration of the gastric mucosa in the rat. Anat Rec 131:193–211
Lawson HH (1970) The origin of chief and parietal cells in regenerating gastric mucosa. Brit J Surg 57:139–141
Lipkin M (1965) Cell replacement in the gastrointestinal tract of man. Gastroenterol 48:616–624
Messier B, Leblond CP (1960) Cell proliferation and migration as revealed by autoradiography after injections of thymidine-H3 into male rats and mice. Am J Anat 106:247–285
Melnyk CS, Brancher RE, Kirsner JB (1967) Regeneration of the human colon mucosa. Morphological and histochemical study. Gastroenterol 52:985–997
Myren J, Torgersen O (1960) Regeneration of parietal cells following experimentally produced gastric ulcers in mice. Acta Pathol Microbiol Scand 60:145–155
Myhre E (1956) Regeneration of the fundic mucosa in rats. Arch path 62:30–36
Townsend SF (1961a) Parenchymal cell origin in regenerating gastric mucosa. Anat Rec 139:280–281
Townsend SF (1961b) Regeneration of gastric mucosa in rats. Am J Anat 109:133–141
Wanke M (1971) Magen. In: Doerr W, Seifert G, Uehlinger E (eds) Spezielle pathologische Anatomie, Bd 2/1, Springer, Berlin Heidelberg New York
Willems G, Galand P, Vansteenkiste Y, Zaiton P (1972) Cell population kinetics of zymogen and parietal cells in the stomach of mice. Z Zellforsch 134:505–518
Williams AW (1961) The healing of experimental gastric ulcers. Brit J Surg 48:564–572
Yamada S, Tsubouchi S (1976) Rapid cell death and cell population recovery in mouse skin epidermis after freezing. Cryobiology 13:317–327
Author information
Authors and Affiliations
Additional information
Dedicated to Professor Dr. W. Maurer on the occasion of his 75th birthday
This work was supported by the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg, He 537/5
Scholar of the Alexander von Humboldt-Foundation
Rights and permissions
About this article
Cite this article
Helpap, B., Hattori, T. & Gedigk, P. Repair of gastric ulcer. Virchows Arch. A Path. Anat. and Histol. 392, 159–170 (1981). https://doi.org/10.1007/BF00430818
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00430818