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The roles of enteric bacterial sialidase, sialateO-acetyl esterase and glycosulfatase in the degradation of human colonic mucin

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Abstract

Sialidase activity in normal faecal extracts showed a preference for mucin-related glycoprotein and oligosaccharide substrates, but the presence of two or moreO-acetyl esters at positions C7–C9 on the sialic acids retarded the rate of hydrolysis. A specific sialateO-acetyl esterase was detected with a lower total activity relative to sialidase with mucin substrates and having a pH optimum of 7.8 and aK M of approximately 1mm sialateO-acetyl ester. A specific glycosulfatase activity was found in faecal extracts using the substrate lactit-[3H]ol 6-O-sulfate with a pH optimum of pH 5.0 and aK M of approximately 1mm.

Faecal extracts from ulcerative colitis (UC) patients had higher sialateO-acetyl esterase and glycosulfatase activity, while mucin sialidase activity was unchanged.

Metabolically labelled mucin isolated from UC patients contained less sulfate and had lower sialic acidO-acetylation compared with normal mucin. Colonic mucin was degraded more efficiently by faecal extracts from UC patients compared with normal extracts. The UC mucin was degraded more rapidly than the normal mucin by faecal enzyme extracts from both normal and UC subjects.

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References

  1. Allen A, Hoskins LC (1988) InDiseases of the Colon, Rectum and Anal Canal (Kirsner JB, Shorter RG, eds) pp. 65–94. Baltimore: Williams and Wilkins.

    Google Scholar 

  2. Allen A, Hutton DA, Pearson JP, Sellars LA (1990) InThe Cell Biology of Inflammation in the Gastrointestinal Tract (Peters TJ, ed) pp. 113–25. Hull, UK: Corners Publications.

    Google Scholar 

  3. Smith AC, Podolsky DK (1986)Clinics Gastroenterol 15: 815–37.

    Google Scholar 

  4. Rogers CM, Cooke KB, Filipe MI (1978)Gut 19: 587–92.

    Google Scholar 

  5. Reid PE, Culling CF, Dunn WL, Clay MG (1984)Histochem J 16: 235–51.

    Google Scholar 

  6. Hutchins JT, Reading CL, Giavazzi R, Hoaglund J, Jessup JM (1988)Cancer Res 48: 483–9.

    Google Scholar 

  7. Filipe MI (1989) InGastrointestinal and Oesophageal Pathology (Whitehead R, ed) pp. 65–89. Edinburgh: Churchill-Livingston.

    Google Scholar 

  8. Rhodes JM, Black RR, Gallimore R, Savage A (1985)Gut 26: 1313–8.

    Google Scholar 

  9. Podolsky DK, Isselbacher KJ (1983)J Clin Invest 72: 142–53.

    Google Scholar 

  10. Corfield AP, Wagner SA, Clamp JR, Kriaris MS, Hoskins LC (1992)Infect Immun 60:3971–78.

    Google Scholar 

  11. Corfield AP, Williams AJK, Clamp JR, Wagner SA, Mountford RA (1988)Clin Sci 74: 71–8.

    Google Scholar 

  12. Corfield AP, Michalski J-C, Schauer R (1981) InPerspectives in Inherited Metabolic Diseases (Tettamanti G, Durand P, DiDonato S, eds) Vol. 4, pp. 3–70. Milan: Edi Ermes.

    Google Scholar 

  13. Corfield AP (1992)Glycobiology 2:509–21.

    Google Scholar 

  14. Corfield AP, Sander-Weaver M, Veh RW, Wember M, Schauer R. (1986)Hoppe-Seylers Biol Chem 367: 433–9.

    Google Scholar 

  15. Corfield AP, Wagner SA, Clamp JR (1987)Biochem Soc Trans 15: 1089.

    Google Scholar 

  16. Wilkinson RK, Roberton AM (1988)FEMS Microbiol Letts 50: 195–9.

    Google Scholar 

  17. Tsai HH, Sunderland D, Gibson GR, Hart CA, Rhodes JM (1992)Clin Sci 82: 447–54.

    Google Scholar 

  18. Hoskins LC, Boulding ET, Gerken TA, Harouny VR, Kriaris MS (1992)Microb Ecology Dis (in press).

  19. Corfield AP, Clamp JR, Wagner SA (1985)Biochem J 226: 163–74.

    Google Scholar 

  20. Veh RW, Michalski J-C, Corfield AP, Sander-Wewer M, Gies D, Schauer R (1981)J Chromatogr 212: 313–22.

    Google Scholar 

  21. Clamp JR, Cooper B, Creeth JM, Ene D, Barrett J, Gough M (1983)Biochem J 215: 421–3.

    Google Scholar 

  22. Lloyd AG (1962)Biochem J 83: 445–60.

    Google Scholar 

  23. Reuter G, Pfeil R, Stoll S, Schauer R, Kamerling JP, Versluis C, Vliegenthart JFG (1983)Eur J Biochem 134: 139–43.

    Google Scholar 

  24. Montreuil J (1982)Compr Biochem 19B(II): 1–188.

    Google Scholar 

  25. Corfield AP, Clamp JR, Aldis J (1987)Biochem Soc Trans 15: 392.

    Google Scholar 

  26. DeVries AL, Vandenheede J, Feeney RE (1971)J Biol Chem 246: 305–8.

    Google Scholar 

  27. Schauer R (1978)Methods Enzymol 50: 64–89.

    Google Scholar 

  28. Corfield AP, Paraskeva C (1992) InMethods in Molecular Biology (Hounsell EF, ed) Vol. 14 New Jersey: Humana Press.

    Google Scholar 

  29. Varki A, Diaz S (1984)Anal Biochem 137: 236–47.

    Google Scholar 

  30. Corfield AP, do Amaral Corfield C, Veh RW, Wagner SA, Clamp JR, Schauer R (1991)Glycoconjugate J 8: 330–9.

    Google Scholar 

  31. Poon H, Reid PE, Ramey CW, Dunn WL, Clay MG (1983)Can J Biochem Cell Biol 61:868–74.

    Google Scholar 

  32. Uchida Y, Tsukada Y, Sugimori T (1979)J Biochem (Tokyo)86: 1573–85.

    Google Scholar 

  33. Rhodes JM, Gallimore R, Elias E, Allan RN, Kennedy JF (1985)Gut 26: 761–5.

    Google Scholar 

  34. Corfield AP, Warren BF, Bartolo DCC, Wagner SA, Clamp JR (1992)Brit J Surg 79:1209–12.

    Google Scholar 

  35. Corfield AP, do Amaral Corfield C, Wagner SA, Warren BF, Mountford RA, Bartolo DCC, Clamp JR (1992)Biochem Soc Trans 20: 95S.

  36. Corfield AP, Wagner SA, Paraskeva C, Clamp JR, Durdey P, Reuter G, Schauer R (1992)Biochem Soc Trans 20: 94S.

    Google Scholar 

  37. Schauer R (1982) InSialic Acids Chemistry, Metabolism and Function (Schauer R, ed) pp. xv-xvii. Vienna: Springer-Verlag.

    Google Scholar 

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Abbreviations: UC, ulcerative colitis; BSM, bovine submandibular gland mucin; PMSF, phenylmethylsulfonyl-fluoride. Sialic acids are abbreviated according to Schauer [37].

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Corfield, A.P., Wagner, S.A., O'Donnell, L.J.D. et al. The roles of enteric bacterial sialidase, sialateO-acetyl esterase and glycosulfatase in the degradation of human colonic mucin. Glycoconjugate J 10, 72–81 (1993). https://doi.org/10.1007/BF00731190

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  • DOI: https://doi.org/10.1007/BF00731190

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