Abstract
The prevalence ofHelicobacter pylori (HP) in the gastric mucosa of patients with chronic atrophic gastritis has been reported to be significantly higher than in normal mucosa. To clarify the role of HP in the etiology of chronic atrophic gastritis, we assessed the effect of ammonia on the gastric mucosal structure in rats, since HP has a strong urease activity and produces abundant amounts of ammonia. Ammonia administered orally at 0.01% and 0.1% as drinking water for two to four weeks decreased the mucosal thickness and the parietal cell number and oxyntic gland number in a dose- and time-dependent manner. The decrease of mucosal thickness was significantly greater in the antral mucosa than in the body mucosa. The border between the antral and body mucosa shifted toward the cardia, reflecting the decrease in oxyntic gland numbers. Furthermore, intracellular mucin was also decreased in a dose- and time-dependent manner, especially in the antral mucosa. Thus, ammonia chronically administered orally in rats led to changes in gastric mucosal structures and functions. The results suggest that the ammonia produced by HP partly plays an etiologic role in chronic atrophic gastritis.
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References
Warren JR, Marshall BJ: Unidentified curved bacilli on gastric epithelium in acute chronic gastritis. Lancet 1:1273–1275, 1983
Marshall BJ, Warren JB: Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet 6:1311–1314, 1984
Buck GE, Gourley WK, Lee WK, Surramanyan K, Latimer JM, DiNuzzo AR: Relation of Campylobacter pyloridis to gastritis and peptic ulcer. J Infect Dis 153:664–669 1986
Dixon MF, Wyatt JI, Burke DA, Rathbone BJ: Lymphocytic gastritis-relationship toCampylobacter pylori infection. J Pathol 154:125–132, 1988
Aase S, Elgio K, Fause O, Bjorneklett A, Nedenskov-Sorensen P, Bukholm G: Bacteris of the gastric antrum and their relation to chronic gastritis. Acta Pathol Microbiol Immunol Scand 96:273–279, 1988
Glupczynski Y, Burette A, Labbe M, Deprez C. Peuch MD, Deltenre M:Campylobacter pylori associated gastritis. A double blind placebo controlled trial with amoxycillin. Am J Gastroenterol 83:365–370, 1988
Rokkas T, Pursey C, Uzoechina E, Dorrington L, Simmons NA, Filipe MI, Sladen GE:Campylobacter pylori and nonulcer dyspepsia. Am J Gastroenterol 82:1149–1152, 1987
Rollason TP, Stone J, Rhodes JM: Spiral organisms in endoscopic biopsies of the human stomach. J Clin Pathol 37:23–26, 1984
McNulty CA, Watson DM: Spiral bacteria of the gastric antrum. Lancet 1:1068–1069, 1984
Langenberg ML, Tytgat GN, Schipper ME, Rietra PJ, Zanen HC:Campylobacter-like organisms in the stomach of patients and healthy individuals. Lancet 1:1348–1349, 1984
Matsui K, Tamura K, Terada A, Murai M, Yamamoto A, Masuzawa M, Fukui O, Abe H, Tanaka M, Funahashi H, Kawano S, Sato N, Kamada T:Campylobacter pylori and gastroduodenal diseases. Jpn J Gastroenterol 84:1864, 1987
Grarpe H, Unge P, Blomqvist C, Makitalo S: Campylobacter pylori in swedish patients referred for gastroscopy. Acta Pathol Microbiol Immunol Scand 96:128–132, 1988
Tsujii M, Kawano S, Ogihara T, Tsujii S, Tanimura H, Hijioka T, Sakura H, Ito T, Hayashi N, Eguchi H, Matsunaga T, Sato N, Kamada T:Campylobacter pylori and PAS-Alcian blue-positive mucin. Gastroenterol Endosc 31:682–687, 1989
Kochiyama T: Cliical study ofCampylobacter pylori in stomach disease. Gastroenterol Endosc 31:3–13, 1989
Kimura K: Chronological transition of the fundic-pyloric border determined by stepwise biopsy of the lesser and greater curvatures of the stomach. Gastroenterology 63:584–592, 1972
Raskov H, Clanng C, Gaarslev K, Fischer Hansen B, Hauch O: Screening forCampylobacter pyloridis in patients with upper dyspepsia and the relation to inflammation of human gastric antrum. Scand J Gastroenterol 22:568–572, 1987
Johnston BJ, Reed PI, Ali MH:Campylobacter-like organism in duodenal and antral endoscopic biopsies: Relationship to inflammation. Gut 27:1132–1137, 1986
Rauws EAJ, Langenberg W, Houthoff HJ, Zanen HC, Tytgat GNJ:Campylobacter pyloridis-associated chronic active antral gastritis. Gastroenterology 94:34–40, 1988
Tsujii M, Kawano S, Sato N, Kamada T: A possible mechanism ofCampylobacter pylori induced mucosal damage. InCampylobacter pylori and Gastroduodenal Diseases. Takemoto T, Kawai K, Shimoyama T (eds). Tokyo, Organizing Committee of Tokyo International Symposium on Campylobacter Pylori, 1989 pp 178–183
Rathbone BJ, Wyatt JI, Heatley RV:Campylobacter pyloridis-a new factor in peptic ulcer disease? Gut 27:635–641, 1986
Tsujii M, Kawano S, Sato N, Kamada T:Campylobacter pylori infection decreases the intracellular mucin in human gastric mucosa. Gastroenterology 96:A517, 1989
Solmiany BL, Bilski J, Sarosick J, Murty VLN, Dworkin B, Van Horn K, Zielenski J, Slomiany A:Campylobacter pyloridis degrades mucin and undermines gastric mucosal integrity. Biochem Biophys Res Commun 144:307–314, 1987
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Kawano, S., Tsujii, M., Fusamoto, H. et al. Chronic effect of intragastric ammonia on gastric mucosal structures in rats. Digest Dis Sci 36, 33–38 (1991). https://doi.org/10.1007/BF01300084
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DOI: https://doi.org/10.1007/BF01300084