Skip to main content
SpringerLink
Log in

Isolierte menschliche Magenschleimhautzellen — Untersuchungen physiologischer und pharmakologischer Regulationsmechanismen

Isolated cells from human gastric mucosa — studies on physiological and pharmacological regulation

  • Originalien
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

Cells were isolated by use of collagenase, EDTA and pronase form human gastric mucosa obtained at peptic ulcer surgery (n=61) or at Whipple's operations (n=6). Enriched parietal cell fractions were prepared by isopycnic centrifugation with Percoll. H+ production, intracellular instrinsic factor and histamine content were maximal in the low density fraction containing 75% parietal cells and — among other nonparietal cell types — mast cells. H+ production, intrinsic factor secretion and adenylate cyclase-activity responded to histamine stimulation in a concentration dependent manner. Response was blocked by histamine H2 receptor antagonists (rantidine, famotidine). Dibutyryl cAMP and the phosphodiesterase inhibitor IMX were the most powerful stimuli whereas carbachol, hexoprenaline and pentagastrin were less effective. Prostaglandin E2 and 6-keto-PGF2α occurred in the highest concentrations in the low density cell fraction. PG production increased linearly for 15 min and seemed to be influenced by the intracellular calcium level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

cAMP:

Adenosin-3',5'-cyclisches Monophosphat

EDTA:

Ethylen Diamin Tetraacetat

IMX:

3-Isobutyl-1-Methylxanthin

Literatur

  1. Batzri S (1981) Direct action of somatostatin on dispersed mucosal cells from guinea-pig stomach. Biochim Biophys Acta 677:521–524

    Google Scholar 

  2. Gottlieb Ch, Lau KS, Wasserman R, Herbert V (1965) Rapid charcoal assay for intrinsic factor (IF), gastric juice unsaturated B12 binding capacity, antibody to IF and serum unsaturated B12 binding capacity. Blood 25:875–883

    Google Scholar 

  3. Lorenz W, Benesch L, Barth H, Matejka E, Meyer R, Kusche J, Hutzel M, Werle E (1970) Fluorometric assay of histamine in tissues and body fluids: choice of the purification procedure and identification of the nanogram range. Z Anal Chem 252:94–98

    Google Scholar 

  4. Miller ThA (1983) Protective effects of prostaglandins against gastric mucosal damage: current knowledge and proposed mechanisms. Am J Physiol 245:G601–623

    Google Scholar 

  5. Ruoff HJ, Becker M (1982) Histamine-sensitive adenylate cyclase in human gastric mucosa: Cellular localization and interaction by PGE2, somatostatin and secretin. Agents and Actions 12:174–175

    Google Scholar 

  6. Ruoff HJ, Wagner M, Günther Ch, Maśliński S (1982) Adrenergic stimulation of isolated rat gastric mucosal cells. Effect on adenylate cyclase and14C-aminopyrine uptake. Naunyn Schmiedeberg's Arch Pharmacol 320:175–181

    Google Scholar 

  7. Sanders MJ, Amirian MA, Ayalon A, Soll AH (1983) Regulation of pepsinogen release from canine chief cells in primary monolayer culture. Am J Physiol 245:G641–646

    Google Scholar 

  8. Schepp W, Ruoff HJ, Maśliński S (1983) Aminopyrine accumulation of isolated parietal cells from the rat stomach. Effect of histamine and interaction with endogenous inhibitors. Arch Int Pharmacodyn Ther 265:293–308

    Google Scholar 

  9. Schepp W, Ruoff HJ, Miederer SE (1983) Cellular origin and release of intrinsic factor from isolated rat gastric mucosal cells. Biochim Biophys Acta 763:426–433

    Google Scholar 

  10. Soll AH (1981) Physiology of isolated canine parietal cells: receptors and effectors regulating function. Aus: Johnson LR (ed) Physiology of the gastrointestinal tract. Raven Press, New York, pp 673–691

    Google Scholar 

  11. Soll AH (1980) Secretagogue stimulation of14C aminopyrine accumulation by isolated canine parietal cells. Am J Physiol 238:G366-G375

    Google Scholar 

  12. Soll AH (1982) Potentiating interactions of gastric stimulants on14C aminopyrine accumulation by isolated canine parietal cells. Gastroenterology 83:216–223

    Google Scholar 

  13. Soll AH, Amirian DA, Thomas LP, Reedy TJ, Elashoff JD (1984) Gastrin receptors on isolated canine parietal cells. J Clin Invest 73:1434–1447

    Google Scholar 

  14. Soll AH, Park J, Thomas LP, Yamada T (1984) Release of somatostatin-like immunoreactivity from canine fundic mucosal cells in primary culture. Am J Physiol 247:G558-G566

    Google Scholar 

  15. Sonnenberg A, Berglindh T, Lewin MJM, Fischer JA, Sachs G, Blum A (1979) Stimulation of acid secretion in isolated gastric cells. In: Rosselin G, Fromageot P, Bonfils S (eds) Hormone receptors in digestion and nutrition. Elsevier/North Holland Biomedical Press, Amsterdam, pp 337–348

    Google Scholar 

  16. Thompson WJ, Jacobson ED, Rosenfeld GC (1982) A role of cyclic AMP in the gastrointestinal tract: receptor control of hydrogen ion secretion by mammalian gastric mucosa. In: Kebabian JW, Nathanson JA (eds) Handbook of Experimental Pharmacology. Springer, Berlin Heidelberg New York, pp 651–682

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Klinik rechts der Isar, Medizinische Klinik II der Technischen Universität München, Germany

    W. Schepp

  2. Medizinische Poliklinik der Universität Bonn, Germany

    W. Schepp & S. E. Miederer

  3. Pharmakologisches Institut der Universität Tübingen, Germany

    H. J. Ruoff

  4. Anatomisches Institut der Universität Bonn, Germany

    U. Wulfhekel

Authors
  1. W. Schepp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. E. Miederer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. J. Ruoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. U. Wulfhekel
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Für diese Studie hat der Bundesminister für Jugend, Familie und Gesundheit den Forschungspreis zur Einschränkung und zum Ersatz von Tierversuchen zuerkannt. Die experimentellen Arbeiten wurden an der Medizinischen Poliklinik der Universität Bonn durchgeführt. Diese Studie wurde durch die Deutsche Forschungsgemeinschaft unterstützt. Wir danken B. Schiermeyer, R. Maier und W. Beer für ihre hervorragende Hilfe als Technische Assistenten

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schepp, W., Miederer, S.E., Ruoff, H.J. et al. Isolierte menschliche Magenschleimhautzellen — Untersuchungen physiologischer und pharmakologischer Regulationsmechanismen. Klin Wochenschr 64, 15–22 (1986). https://doi.org/10.1007/BF01721576

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01721576

Key words

Search

Navigation