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Determination of histidine decarboxylase mRNA in various rat tissues by the polymerase chain reaction

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Abstract

Histidine decarboxylase (HDC) mRNA in various rat tissues were quantitated by using a reverse transcription-polymerase chain reaction (RT-PCR) in which a mouse mRNA was used as an internal standard. The stomach HDC mRNA level was the highest followed by the brain, skin, jejunum, spleen and liver. There was no measurable HDC mRNA in the kidney. The stomach HDC activity was also the highest followed by the brain, skin, spleen, jejunum, liver and kidney. A significant correlation (r = 0.940,p < 0.0001) was observed between the HDC mRNA levels and HDC activities in these tissues. We have also examined the HDC mRNA levels in fasting rats and found that HDC mRNA levels in the stomach were reduced after the 48-hr-fasting with the decrease in HDC activities. These observations indicate that there may exist a gene regulation, at least at the basal level, for the HDC activities in the rats.

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References

  1. Dimaline R, Sandvik AK. Histidine decarboxylase gene expression in rat fundus is regulated by gastrin. FEBS Lett 1991;281:20–2.

    PubMed  Google Scholar 

  2. Gilliand G, Perrin S, Blanchard K, Bunn HF. Analysis of cytokine mRNA and DNA; detection and quantitation by competitive polymerase chain reaction. Proc Natl Acad Sci USA 1991;87:2725–9.

    Google Scholar 

  3. Chelly J, Kaplan JC, Maire P, Gautron S, Kahn A. Transcription of the dystrophin gene in human muscle and non-muscle tissues. Nature (London) 1988;333:858–60.

    Google Scholar 

  4. Noonan KE, Beck C, Holzmayer TA, Chin JE, Wunder JS, Andrulis IL, Gazdar AF, Willman CL, Griffith B, Von Hoff DD, Roninson IB. Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc Natl Acad Sci USA 1990;87:7160–4.

    PubMed  Google Scholar 

  5. Wang AM, Doyle MV and Mark DF. Quantitation of mRNA by the polymerase chain reaction. Proc Natl Acad Sci USA 1989;86:9717–21.

    PubMed  Google Scholar 

  6. Becker-André M, Hahlbrock K. Absolute mRNA quantitification using the polymerase chain reaction (PCR); a novel approach by a PCR aided transcript titration assay (PATTY). Nucleic Acids Res 1989;17:9437–46.

    PubMed  Google Scholar 

  7. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987;162:156–9.

    PubMed  Google Scholar 

  8. Joseph DR, Sullivan PM, Wang Y-M, Kozak C, Fenstermacher DA, Behrendsen ME, Zahnow CA. Characterization and expression of the complementary DNA encoding rat histidine decarboxylase. Proc Natl Acad Sci USA 1990;87:733–7.

    PubMed  Google Scholar 

  9. Yamamoto J, Yatsunami K, Ohmori E, Sugimoto Y, Fukui T, Katayama T, Ichikawa A. cDNA-derived amino acid sequence of L-histidine decarboxylase from mouse mastocytoma P-815 cells. FEBS Lett 1990;276:214–8.

    PubMed  Google Scholar 

  10. Miyazaki Y, Imamura I, Shinomura Y, Wada H, Tarui S. Marked increase in gastric histidine decarboxylase activity in patients with hypergastrinemia. Life Sciences 1991;48:51–8.

    PubMed  Google Scholar 

  11. Yamatodani A, Fukuda H, Wada H. High-performance liquid chromatographic determination of plasma and brain histamine without previous purification of biological samples; cation-exchange chromatography coupled with post-column derivatization fluorometry. J Chromatogr 1985;344:115–23.

    PubMed  Google Scholar 

  12. Yamada M, Watanabe T, Harino S, Fukui H, Wada H. The effects of protease inhibitors on histidine decarboxylase activities and assay of enzyme in various rat tissues. Biochim Biophys Acta 1980;615:458–64.

    PubMed  Google Scholar 

  13. Gill DS, Thompson CS, Dandona P. Histamine synthesis and catabolism in various tissues in diabetic rats. Metabolism 1990;39:815–8.

    PubMed  Google Scholar 

  14. Fisher DZ, Meier E, Fitzpatrick D. Histamine, free-histidine concentrations and histidine decarboxylase, histamine methyl-transferase activities in developing rat tissues. Comp Biochem Physiol 1981;68C:231–4.

    Google Scholar 

  15. Lorenz W, Halbach St., Gerant M, Werle E. Specific histidine decarboxylases in the gastric mucosa of man and other mammals. Biochem Pharmacol 1969;18:2625–37.

    PubMed  Google Scholar 

  16. Neugebauer E, Lorenz W. A modified Schayer procedure for the estimation of histidine decarboxylase activity: Its application on tissue extracts from gastric mucosa of various mammals. Agents Actions 1982;12:32–40.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacology II, Osaka University Medical School, 565, Suita, Japan

    I. Imamura & H. Fukui

  2. Second Department of Internal Medicine, Osaka University Medical School, 565, Suita, Japan

    S. Kondo, I. Imamura, Y. Shinomura, Y. Matsuzawa & H. Fukui

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  1. S. Kondo
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  2. I. Imamura
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  3. Y. Shinomura
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  4. Y. Matsuzawa
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  5. H. Fukui
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Kondo, S., Imamura, I., Shinomura, Y. et al. Determination of histidine decarboxylase mRNA in various rat tissues by the polymerase chain reaction. Inflamm Res 44, 111–115 (1995). https://doi.org/10.1007/BF01782020

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  • DOI: https://doi.org/10.1007/BF01782020

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