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Changes in colonic tissue levels of inflammatory mediators in a guinea-pig model of immune colitis

  • Immunosupression and Inflammation
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Abstract

An immune colitis based on a delayed-type hypersensitivity reaction was induced in dinitrochlorobenzene (DNCB)-sensitized guinea-pigs by intrarectal challenge with DNCB. A low challenge dose (0.25% DNCB) induced mild inflammatory changes in the distal colon and rectum characterized by goblet cell depletion. A higher challenge concentration (5% DNCB) resulted in severe colonic ulceration with crypt abscess formation. The inflammatory mediators, histamine, 5-hydroxytryptamine (5HT), glandular kallikrein and PGE2 were measured in freeze-dried colonic mucosae. Histamine content was three times control (p<0.01) in 0.25% DNCB induced colitis, although no significant change was observed in 5% DNCB challenged animals. Mucosal 5HT content was significantly reduced (p<0.01) after both challenges. Glandular kallikrein content did not differ from control, while PGE2 was significantly (p<0.05) increased at both challenge doses. The possible significance of these changes with respect to severity of inflammation and aetiology of colitis is discussed.

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Author notes

  1. A. A. Norris

    Present address: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, N1G 2W2, Guelph, Ontario, Canada

Authors and Affiliations

  1. Department of Physiology and Pharmacology, University of Strathclyde, Glasgow, UK

    A. A. Norris & I. J. Zeitlin

  2. Organon Labs Ltd, Newhouse, Lanarkshire, UK

    A. J. Lewis

Authors
  1. A. A. Norris
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  2. A. J. Lewis
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  3. I. J. Zeitlin
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Norris, A.A., Lewis, A.J. & Zeitlin, I.J. Changes in colonic tissue levels of inflammatory mediators in a guinea-pig model of immune colitis. Agents and Actions 12, 243–246 (1982). https://doi.org/10.1007/BF01965154

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  • DOI: https://doi.org/10.1007/BF01965154

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