Abstract
The morphological changes in the intestinal tunica muscularis induced by prolonged dietary fiber intake were determined in rat small intestine and colon with the aid of computerized image analysis. Thirty male Sprague-Dawley rats were fed either a fiber free, 15% cellulose or 15% pectin diet for 8 weeks. Intestine length was measured and stained cross sections of the jejunum, ileum, and colon were quantitated using image analysis. In the distal colon, muscle cell size was also determined. Despite lower weight gain in the pectin fed rats, both the small intestine and colon length were significantly increased. Cellulose feeding had a lesser effect on intestine length. Pectin fed rats had significantly increased relative tunica muscularis area (37.2±2.2 mm2) in ileum cross sections when compared to control (24.3±1.8 mm2) and cellulose fed rats (26.1±1.1 mm2). In the mid-colon, the tunica muscularis area was found to be pectin > cellulose > control (33.5±2.2; 29.7±1.7; 25.8±1.5 respectively) with significant differences reached between pectin and control rats. In jejunal samples, no differences were observed among the groups. Circular smooth muscle cell size in the distal colon was significantly increased following cellulose feeding but was less pronounced in the case of pectin. We conclude that fiber supplementation leads to morphological changes in the rat intestine including changes in length and tunica muscularis volume.
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Dreher ML: Dietary Fiber and its physiological effects.In Handbook of Dietary Fiber—An Applied Approach. New York, Marcel Dekker, 1987, pp 199–279
Cassidy MM, Lightfoot FG, Grau LE, Story JA, Kritchevsky D, Vahouny GV: Effect of chronic intake of dietary fibers on the ultrastructural topography of rat jejunum and colon: A scanning electron microscopy study. Am J Clin Nutr 34:218–228, 1981
Vahouny GV, Cassidy MM: Dietary fiber and intestinal adaptation.In Dietary Fiber—Basic and Clinical Aspects. GV Vahouny, D Kritchevsky (eds). New York, Plenum Press, 1986, pp 181–210
Jacobs LR, Schneeman BO: Effects of dietary wheat bran on rat colonic structure and mucosal cell growth. J Nutr 111:798–803, 1981
Jacobs LR: Effects of dietary fiber on mucosal growth and cell proliferation in the small intestine of the rat: A comparison of oat bran, pectin, and guar with total fiber deprivation. Am J Clin Nutr 37:954–960, 1983
Brown RC, Kelleher J, Losowsky MS: The effect of pectin on the structure and function of the rat small intestine. Br J Nutr 42:357–365, 1979
Jacobs LR, White FA: Modulation of mucosal cell proliferation in the intestine of rats fed a wheat bran diet. Am J Clin Nutr 37:945–953, 1983
Judd PA, Truswell AS: The hypocholesterolaemic effects of pectins in rats. Br J Nutr 53:409–425, 1985
Bertram T, Henderson C, Argenzio R, Lawrence A, Sutton J: Adaptive response of the F-344 rat large intestine to dietary fiber. Vet Pathol 29:456, 1992
Sandberg A, Ahderinne R, Andersson H, Hallgren B, Hulten L: The Effect of citrus pectin on the absorption of nutrients in the small intestine. Hum Nutr Clin Nutr 37C:171–183, 1983
Isaksson G, Asp NG, Ihse I: Effect of dietary fibre on pancreatic enzyme activities of ileostomy evacuates and on excretion of fat and nitrogen in the rat. Scand J Gastroenterol 18:417–423, 1983
Schwartz SE, Levine GD: Effects of dietary fibre on intestinal glucose absorption and glucose tolerance in rats. Gastroenterology 79:833–836, 1980
Jenkins DJA, Wolever TMS, Leeds AR, Gassull MA, Haisman D, Dilawari J, Goff D, Metz GL, Alberti KGMM: Dietary fibres, fibre analogues and glucose tolerance: Importance of viscosity. Br Med J 1:1392–1394, 1978
Anderson JW, Midgley WR, Wedman B: Fibre and diabetes. Diabetes Care 2:369–379, 1979
Schneeman BO, Richter D: Changes in plasma and hepatic lipids, small intestinal histology and pancreatic enzyme activity due to aging and dietary fiber in rats. J Nutr 123:1328–1337, 1993
Bertram TA: Gastrointestinal Tract.In Handbook of Toxicologic Pathology. WM Haschek, CG Rousseaux (eds). San Diego, Academic Press, 1991, pp 195–251
Wrick KL, Robertson JB, Van Soest PJ, Lewis BA, Rivers JM, Roe DA, Hackler LR: The influence of dietary fiber source on human intestinal transit and stool output. J Nutr 113:1464–1479, 1983
Anderson JW, Deakins DA, Bridges SR: Soluble fiber—hypocholesterolemic effects and proposed mechanisms.In Dietary Fiber, Chemistry, Physiology, and Health Effects. D Kritchevsky, C Bonfield, JW Anderson (eds). New York, Plenum Press, 1990, pp 339–364
Jacobs LR: Differential effects of dietary fibers on rat intestinal circular muscle cell size. Dig Dis Sci 30:247–252, 1985
Smith HA, Jones TC, Hunt RD: Disturbances of growth.In Veternary Pathology. Philadelphia, Lea & Febiger, 1972, pp90–103
Murphy RA: Muscle as a tissue.In Physiology. RM Berne, MN Levy (eds). St. Louis, CV Mosby, 1988, pp 343–355
Greaves P: Digestive System 1.In: Histopathology of Preclinical Toxicity Studies: Interpretation and Relevance in Drug Safety Evaluation. Amsterdam, Elsevier, 1990, pp 278–392
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Support for this research was partially provided by: The Ben Gurion Fund for the Encouragement of Research Histadrut—The General Federation of Labor in Israel.
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Stark, A., Nyska, A., Zuckerman, A. et al. Changes in intestinal tunica muscularis following dietary fiber feeding in rats. Digest Dis Sci 40, 960–966 (1995). https://doi.org/10.1007/BF02064183
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DOI: https://doi.org/10.1007/BF02064183