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Protective effects of endothelin-1 on acute pancreatitis in rats

  • Pancreatic And Bitiary Disorders
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Abstract

Endothelin-1, a 21-residue peptide isolated from vascular endothelial cells, has a broad spectrum of actions. To clarify the involvement of endothelin-1 in acute pancreatitis, we examined the effects of endothelin-1 and its receptor antagonist BQ-123 on cerulein-induced pancreatitis in rats. Rats were infused intravenously with heparin-saline (control), endothelin-1 (100 pmol/kg/hr), cerulein (5 µg/kg/hr), or cerulein plus endothelin-1 for 3.5 hr. In another experiment, cerulein or cerulein plus BQ-123 (3 mg/kg/hr) was infused. Infusion of cerulein caused hyperamylasemia and pancreatic edema. Endothelin-1, when infused with cerulein, decreased the extent of pancreatic edema with a significant increase in the pancreatic dry- to wet-weight ratio. Histological changes induced by cerulein were markedly attenuated when endothelin-1 was given with cerulein. In contrast, endothelin-receptor blockade with BQ-123 further augmented pancreatic edema caused by cerulein. The extent of inflammatory cell infiltration was greater when BQ-123 was given with cerulein. Endothelin-1 or BQ-123 had no influence on hyperamylasemia. This study suggests that endothelin-1 has protective effects on experimental acute pancreatitis.

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Authors and Affiliations

  1. From the First Department of Surgery, Faculty of Medicine; and College of Medical Technology, Kyoto University, 606, Kyoto, Japan

    Masafumi Kogire MD, Kazutomo Inoue MD, Shun-Ichi Higashide MD, Kyoichi Takaori MD, Yoshiya Echigo MD, Yuan-Jun Gu MD, Shoichiro Sumi MD, Koutaro Uchida MD & Masayuki Imamura MD

Authors
  1. Masafumi Kogire MD
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  2. Kazutomo Inoue MD
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  3. Shun-Ichi Higashide MD
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  4. Kyoichi Takaori MD
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  5. Yoshiya Echigo MD
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  6. Yuan-Jun Gu MD
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  7. Shoichiro Sumi MD
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  8. Koutaro Uchida MD
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  9. Masayuki Imamura MD
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Supported by a grant from the Ministry of Education, Japan (No. B-04454330).

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Kogire, M., Inoue, K., Higashide, SI. et al. Protective effects of endothelin-1 on acute pancreatitis in rats. Digest Dis Sci 40, 1207–1212 (1995). https://doi.org/10.1007/BF02065525

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  • DOI: https://doi.org/10.1007/BF02065525

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