Skip to main content
SpringerLink
Log in

Long-term observations on morphological changes of choledochal epithelium after choledochoenterostomy in rats

  • Original Articles
  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Morphological changes of the common bile duct were observed macroscopically and microscopically 20 months after choledochojejunostomy and choledochocolonostomy in rats. The common bile ducts were remarkably dilated in all rats of both experimental models. Choledochal stones consisting of fatty acid calcium and calcium bilirubinate were seen in two of six rats with choledochojejunostomy and two of five rats with choledochocolonostomy. The main pathological change observed in both the groups was remarkable hyperplasia of the choledochal epithelium. Only one rat with choledochojejunostomy revealed normal epithelium with choledochal stone formation. Intestinal metaplasia was seen in two rats with choledochojejunostomy and all with choledochocolonostomy. Squamous pseudostratification of the epithelium indicating atypism was observed in two rats with choledochojejunostomy. Sialomucin producing cells and sulfomucin producing cells were seen in the hyperplastic portion of the epithelium. No malignant alteration of the epithelium was detected. These findings indicate that long-lasting exposure to digestive enzymes and bacteria causes epithelial hyperplasia and further exposure to digestive enzymes plays a major role in appearance of the epithelial atypism. Carcinogenesis of the choledochal epithelium under such an environment will need much more time to be established.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Nakamura Y, Ohta G, Nagakawa T, Matsubara F: Pathological findings of hepatolithiasis. Jpn J Gastroenterology 78:874–882, 1981

    Google Scholar 

  2. Komi N, Tamura T, Tsuge S, Miyoshi Y, Udaka H: Relation of patient age to premalignant alterations in choledochal cyst epithelium. J Pediatr Surg 21:430–433, 1986

    Google Scholar 

  3. Helen MS: Occurrence of an adenocarcinoma at the choledochoenteric anastomosis 14 years after pancreatoduodenostomy for benign disease. Gastroenterology 72:322–324, 1977

    Google Scholar 

  4. Eleftheliadis E, Tzioufa V, Kotzampassi K, Aletras H: Common bile-duct mucosa in choledochoduodenostomy patients—histological and histochemical study. HPB Surg 1:15–20, 1988

    Google Scholar 

  5. Okawa H, Sawaguchi S, Yamazaki H, Sakaba M, Ishikawa A: Research on animal models of the anomalous pancreatic-biliary duct union. Jpn J Pediatr Surg 18:185–191, 1982

    Google Scholar 

  6. Ogura Y: Experimental studies on reflux of pancreatic juice to the effects in the hepatobiliary system. Jpn J Surg Soc 84:141–149, 1983

    Google Scholar 

  7. Haratake J, Horie T, Takeda N: Occurrence of an intrapancreatic choledochal carcinoma 9 years after choledochojejunostomy for benign disease. Jpn J Cancer Clin 29:1367–1370, 1983

    Google Scholar 

  8. Yamamoto K, Tsuchiya R, Ito T, Harada S: A study of cholangiocarcinoma coexistent with hepatolithiasis. Jpn J Gastroenterol Surg 17:601–609, 1984

    Google Scholar 

  9. Nakashiro H, Watanabe T, Harada S: Clinicopathological and immunohistological studies of lymph follicular cholecystitis. Jpn J Biliary Assoc 4:197–202, 1990

    Google Scholar 

  10. Madden JL, Chun JK, Kandalaft S: Choledochoduodenostomy—an unjustly maligned surgical procedure? Am J Surg 119:45–54, 1970

    Google Scholar 

  11. Bithmuth H, Franco D, Corlette MB, Hepp J: Long term results of Roux-Y hepaticojejunostomy. Surg Gynecol Obstet 146:161–167, 1976

    Google Scholar 

  12. Shita M, Suzuki T, Manabe T, Uchida K, Tobe T: Cholangitis after biliary reconstruction. Jpn J Surg Soc 87:1079–1083, 1986

    Google Scholar 

  13. Nagai T, Kurumado K, Yamakawa T: The bilioenyeric anastomosis using rats. Jpn J Gastroenterol Surg 218:2354, 1988

    Google Scholar 

  14. Nagai T, Kurumado K, Yamakawa T: Experimental model for screening of carcinogenesis of the biliary tract. Proc Jpn Res Soc Gastroenterol Carcinogen 1:293–296, 1986

    Google Scholar 

  15. Nagai T, Kurumado K, Yamakawa T: Experimental study on changes of biliary tract mucosa after reconstruction of biliary tract. Jpn J Biliary Assoc 4:366, 1990

    Google Scholar 

  16. Kurumado K, Nagai T, Yamakawa T: Usefulness of chole-dochoenterostomy model in surgical research. Jpn J Surg Soc 92:357, 1990

    Google Scholar 

  17. Nagai T, Kurumado K, Yamakawa T, Kondo Y: Experimental study on morphological changes in the common bile epithelium after choledochojejunostomy and choledochocolonostomy. Teikyo Med J 14:463–470, 1991

    Google Scholar 

  18. Lee S, Chalters AC, Chandler JG, Orloff MJ: A technique for orthotopic liver transplantation. Transplantation 16:664–669, 1973

    Google Scholar 

  19. Karnowsky MI: A formaldehyde-glutaraldehyde fixate of high osmolarity for use in electron microscopy. J Cell Biol 27:137A-138A, 1965

    Google Scholar 

  20. Mowry RW: The special value of methods that color both acidic and vicinal hydroxyl group in the technical study of mucin; with revised directions for the colloidal iron stain, the use of Alcian blue G8X and their combinations with the PAS reaction. Ann NY Acad Sci 106:402–423, 1963

    Google Scholar 

  21. Spicer SS: Diamine methods for differentiating mucosubstances histochemically. J Histochem Cytochem 13:211–234, 1965

    Google Scholar 

  22. Yamamoto M, Saito Y, Oyanagi H: Anomalous pancreaticobiliary ductal union associated with biliary tract carcinoma. Surg Ther 56:185–195, 1987

    Google Scholar 

  23. Kuroda K, Wada Y, Atomi Y: Association of carcinoma with congenital dilatation of the bile duct. J Bil Panc 2:1627–1635, 1981

    Google Scholar 

  24. Tamura T, Komi N, Miyoshi Y: Meaning of intestinal meta-plasia in congenital choledochal dilatation, especially in relation with carcinogenesis.In Shokakibyo no Shimpo. Y Ohta (ed). Tokyo, Nihon Igakukan, 1986, pp 296–297

    Google Scholar 

  25. Yamaguchi N, Konishi F, Tanikawa Y: Comparative study on changes of epithelial mucin, CEA, and CA 19-9 distribution in age order and occurrence of carcinoma in biliary tract mucosa of cases with anomalous pancreaticobiliary ductal union.In Shokakibyo no Shimpo. Y Ohta (ed). Tokyo, Nihon Igakukan, 1986, pp 293–295

    Google Scholar 

  26. Nakamura T: Experimental study on the association between bacterial infection and chronic proliferative cholangitis of the intrahepatic bile ducts in dogs. Jpn J Biliary Assoc 5:113–124, 1991

    Google Scholar 

  27. Maki T: Pathogenesis of calcium bilirubinate gallstone; role ofE. coli β-glucuronidase and coagulation by inorganic ions, polyelectrolytes and agitation. Ann Surg 164:90–100, 1966

    Google Scholar 

  28. Matsushiro T, Nemoto T, Endo M: Glycoproteins and sulphated glycoproteins isolated from human bile. Clin Chem Acta 30:645–655, 1970

    Google Scholar 

  29. Lee SP, Lim TH, Scott AI: Carbohydrate moities of glycoproteins in human hepatic and gallbladder mucosa and gallstones. Chin Sci 56:533–538, 1979

    Google Scholar 

  30. Goto A, Onizuka A, Hayashi I, Inui H: Bacteria and β-glucuronidase activity in bile of patients with hepatolithiasis.In Report of Hepatolithiasis Research Committee of Japan. F Nakayama (ed). Ministry of Welfare, Japan, 1982, pp 113–123

    Google Scholar 

  31. Kanai M: Clinical and experimental studies on the formation of calcium bilirubinate gallstones. Jpn J Surg Soc 88:191–197, 1987

    Google Scholar 

  32. Sasaki M, Mori T, Isaka N, Miyagishima K: Role of bile glycoprotein in hepatolithiasis and experimental animal model. Jpn J Biliary Assoc 1:39–48, 1987

    Google Scholar 

  33. Shoda J, Tanaka N, Matsuzaki Y, Osuga T, Shigematsu S, Miyazaki H: Microanalysis of bile acid composition in intrahepatic calculi and its etiological significance. Gastroenterology 101:821–831, 1991

    Google Scholar 

  34. Way LW: Diseases of the gallbladder and bile duct.In Cecil Textbook of Medicine, 17th ed, vol 1. JB Wyngaarden, LH Smith (eds). Philadelphia, Saunders, 1982, pp 752–768

    Google Scholar 

  35. Ockner RK: Diseases of the liver.In Cecil Textbook of Medicine, 17th ed, vol 1. JB Wyngaarden, LH Smith (eds). Philadelphia, Saunders, 1982, pp 769–813

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. From the Department of Surgery, University Hospital, Mizonokuchi, Teikyo University School of Medicine, 74 Mizonokuchi, Takatsu-Ku, Kawasaki, 213, Kanagawa-Ken, Japan

    Katsumaro Kurumado MD, Tetsushi Nagai MD, Yoshio Kondo MD & Hiroyuki Abe BSc

Authors
  1. Katsumaro Kurumado MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tetsushi Nagai MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yoshio Kondo MD
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hiroyuki Abe BSc
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kurumado, K., Nagai, T., Kondo, Y. et al. Long-term observations on morphological changes of choledochal epithelium after choledochoenterostomy in rats. Digest Dis Sci 39, 809–820 (1994). https://doi.org/10.1007/BF02087428

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02087428

Key words

Search

Navigation