Abstract:
The effect of obstructive jaundice on neutrophil chemotactic function was investigated, with a potent chemotactic factor, IL-8 (recombinant rat GRO-β), in rats that received 7-day bile duct ligation. Carrageenin or IL-8 was injected into a preformed air pouch, and exudate was collected 4 h later for measurement of myeloperoxidase activity. In vitro chemotaxis of peripheral neutrophils to IL-8 was evaluated by a modified Boyden chamber method. Both carrageenin and IL-8 induced significantly pronounced intra-air pouch neutrophil recruitment in the bile duct-ligated group compared with a sham-ligated group. In vitro neutrophil chemotaxis was significantly increased in the bile duct-ligated group compared with the sham-ligated group. The present experimental model suggests enhanced neutrophil chemotaxis to IL-8 in obstructive jaundice.
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(Received Sept. 10, 1997; accepted Jan. 23, 1998)
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Yanagitani, K., Kubota, Y., Tsuji, K. et al. Influence of biliary obstruction on neutrophil chemotaxis. J Gastroenterol 33, 536–540 (1998). https://doi.org/10.1007/s005350050128
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DOI: https://doi.org/10.1007/s005350050128