Elsevier

Analytical Biochemistry

Volume 107, Issue 2, 15 September 1980, Pages 424-431
Analytical Biochemistry

Optimization of conditions for the colorimetric determination of citrulline, using diacetyl monoxime

https://doi.org/10.1016/0003-2697(80)90404-2Get rights and content

Abstract

A method is described for colorimetric determination of citrulline following deproteinization, depending upon the reaction with diacetyl monoxime in the presence of sulfuric and phosphoric acids. Ferric chloride is included to sensitize the reaction and thiosemicarbazide to improve light stability of the chromogen. The method offers diminished heating time compared with existing procedures, combined with good sensitivity and photostability of the chromonphore.

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