Elsevier

Clinica Chimica Acta

Volume 207, Issue 3, 15 May 1992, Pages 227-237
Clinica Chimica Acta

Purification and characterisation of antigenic gliadins in coeliac disease

https://doi.org/10.1016/0009-8981(92)90121-6Get rights and content

Abstract

Two gliadins, known to be especially antigenic in coeliac disease, were purified to homogeneity by a series of ion-exchange chromatography steps. Their N-terminal amino acid sequences showed minor differences but clearly classified them as gamma-type gliadins. The purified gliadins were further characterised with respect to amino acid composition, molecular mass and E1cm1% at 276 nm. Based on these properties it is suggested that one of them is identical to a gamma-type gliadin, earlier characterised by its nucleotide sequence, whereas the other has not previously been described. The purification procedure may form the basis for the development of a more differentiated analysis of circulating antibodies for diagnosis and makes clinical testing of the toxicity of defined gliadin peptides feasible.

References (26)

  • JA Rafalski

    Structure of wheat gamma-gliadin genes

    Gene

    (1986)
  • SU Friis et al.

    Patients with coeliac disease have a characteristic gliadin antibody pattern

    Clin Chim Acta

    (1986)
  • L Charbonnier

    Etude des protéines alcoolo-solubles de la farine de blé

    Biochimie

    (1973)
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